Genistein对去卵巢大鼠基底前脑胆碱能神经元影响的体内研究  

Effect of genistein on basal forebrain cholinergic neurons of ovariectomized rats in vivo

作　　者：张志军[1,2] 董玉林[1,2] 郁晓燕[1,2] 何志贤[1,2] 胡燕[1,2] 杨学峰[1,2] 倪衡建[1,2] 

机构地区：[1]南通大学医学院人体解剖学教研室神经生物学研究所 [2]江苏省神经再生重点实验室,南通226001

出　　处：《神经解剖学杂志》2008年第4期367-372,共6页Chinese Journal of Neuroanatomy

摘　　要：本文旨在研究染料木素(genistein)对去卵巢大鼠基底前脑胆碱能神经元的影响。雌性大鼠双侧卵巢切除2周后用genistein和雌激素替代治疗1周。称子宫重量以确定手术是否成功及雌二醇(E2)的治疗是否有效。用免疫组化染色、RT-PCR和Westernblot等方法对胆碱能神经元数量、ChAT基因和蛋白的表达量进行检测。结果显示:去卵巢3周后子宫变轻,雌激素替代治疗能增加去卵巢子宫的重量,而genistein替代治疗对去卵巢子宫的重量影响不明显;去卵巢3周后,内侧隔核(MS)和斜角带垂直臂核(VDB)内的胆碱能神经元数量、ChAT基因和蛋白的表达量均明显减少,雌激素和genistein替代治疗后能显著增加去卵巢大鼠MS和VDB内的胆碱能神经元数量、ChAT基因和蛋白的表达量。本研究结果提示:genistein对去卵巢大鼠基底前脑胆碱能神经元具有类似雌激素样神经保护作用,而对子宫影响不明显。To study the effects of genistein on basal forebrain cholinergic neurons of ovariectomized （OVX） rats. Young female rats were ovariectomized bilaterally and two weeks later, treated with genistein and 17 [3-estradiol （E2） for one week. The weight of uterus was measured and used to confirm the success of ovariectomy and E2 treatment. The cholinergic neurons were detected by immunohistochemical staining for CHAT. The ChAT mRNA and protein were detected by RT-PCR and Western blot, respectively. The results showed that, three weeks later, the OVX and genistein-treated rats bad lower uterine weight than estrogen-treated rats, whereas the estrogen-treated rats and the sham-operated rats had no significant difference in uterine weight. Three weeks later, the number of cholinergic neurons, the expression of ChAT mRNA and protein were significantly reduced in the medial septal nucleus （MS） and nucleus of the vertical limb of the diagonal band （VDB） of OVX rats. E2 and genistein could prevent a decrease in the number of cholinergic neurons, the expression of ChAT mRNA and protein in the MS and VDB of OVX rats. The preseut results indicate that genistein has neuroprotective effect similar to E2 on basal forebrain eholinergie neurons of OVX rats in vivo. Furthermore, the genistein has no significant effect on the uterus of OVX rats.

关 键 词：染料木素 雌激素 胆碱乙酰基转移酶 胆碱能神经元 基底前脑 大鼠 

分 类 号：R285.5[医药卫生—中药学]