orexin A和orexin 1型受体在新生大鼠与成年大鼠延髓分布的比较  

Distribution of orexin A and orexin type 1 receptor in the medulla of neonatal and adult rats

作　　者：宁穗[1] 宋娜娜[1] 刘自兵[1] 李莉[1] 曹银祥[1] 钱源[1] 朱大年[1] 沈霖霖[1] 

机构地区：[1]复旦大学上海医学院生理与病理生理学系,上海200032

出　　处：《神经解剖学杂志》2008年第4期401-405,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(No.30670771);上海市科委基金(No.07OZ19722-3);上海市重点学科建设项目(No.B112)资助项目

摘　　要：本文研究orexinA(OXA)和orexin 1型受体(OX1R)在新生大鼠与成年大鼠延髓的分布及比较。新生(1～5d)和成年(6～8周)SD大鼠,取其延髓,采用免疫组织化学方法和图像分析技术,观察OXA和OX1R在新生与成年SD大鼠延髓的分布。结果显示,OXA免疫反应阳性纤维和OX1R免疫反应阳性细胞在新生和成年SD大鼠延髓内有广泛分布,主要分布在延髓腹外侧区(ventrolateralmedulla,VLM)和舌下神经核(hypoglossalnucleus,XII)。在这两个区域,新生大鼠组的OX1R免疫反应阳性细胞的相对光密度值均低于成年大鼠组(P<0.001)。结果表明随着大鼠发育成熟,OX1R表达水平增加,可能与其生理功能完善有关。OXA免疫反应纤维和OX1R免疫反应阳性细胞在延髓的VLM和XII的表达提示可能与心血管和呼吸等活动的调节有关。Present study investigated the distribution of orexin A-like-immunoreactivity （OXA-like-ir） and orexin type 1 receptor-like-immunoreactivity （OX1 R-like-ir） in the medulla of neonatal and adult Sprague-Dawley （SD） rats. OXA-like-and OX1 R-like-ir were separately observed in the medulla of neonatal （1 -5 d） and adult （6-8 weeks） SD rats by using immunohistochemical techniques and software of ImageMeasure. Expressions of OXA-like-and OX1R-like-ir were observed throughout the medulla of neonatal and adult rats. OXA-like-ir fibers and OXlR-like-ir positive cells were mainly in the ventrolateral medulla （VLM） and hypoglossal nucleus （XII）. In the VLM and XII, relative optical density （ROD） of OX1R-like-ir positive cells in neonatal rats was less than that in adult rats （P〈0.001）. The expression of OX1R-like-ir increased in adult rat may indicate its important role in physiological functions with maturation. The presence of orexinA-like-ir fibers and OX1R-like-Jr neurons in both the XII and VLM is in support of its proposed role in regulation of cardiovascular and respiratory activities,

关 键 词：增食欲素A 增食欲素1型受体 免疫组织化学 延髓 大鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]