基于叶绿体DNA序列分析东亚七筋姑的遗传进化  

Genetic Evolution of Clintonia udensis Trautv.et Mey.Based on cpDNA Sequencing

作　　者：李欣[1] 刘占林[1] 王祎玲[1] 李思锋[2] 赵桂仿[1] 

机构地区：[1]西北大学西部资源生物与现代生物技术教育部重点实验室,西安710069 [2]西安植物园,西安710061

出　　处：《西北植物学报》2008年第6期1112-1117,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：教育部博士点基金项目(20040697010);国家自然科学基金项目(30670129);陕西省自然科学基金项目(2005C117)

摘　　要：通过对东亚七筋姑(Clintoniaudensis Trautv.etMey.)19个居群的cpDNA基因间隔区(cpDNA intergenic regions)rpl20-rps12和trnL-F进行序列分析,结果表明,当空位(Gap)作缺失处理时,trnL-F全序列排序后的长度为754个位点,G+C含量为35.2%～35.8%,rpl20-rps12序列排序后长度为814bp,G+C含量为34.1%～34.3%.聚类结果显示四倍体居群没有单独聚为一支,其多倍体可能是同源多倍体,起源方式属于异地多起源.Sequences of cpDNA intergenic regions （rpl20-rpsl2 and trnL-F）were used for genetic analysis of 19 populations of Clintonia udensis Trautv. et Mey. The results indicated that the lengths of trnL-F and rpl20-rps12 are 754 bp and 814 bp,and the content of G＋C is from 35.2% to 35.8% and from 34.1% to 34.3% respectively when the gaps were treated as missing. The cluster analysis showed that all the tetraploid populations were not clustered, which may indicate that the origin of C. udensis polyploidy was autopolyploidy polytopic.

关 键 词：东亚七筋姑 叶绿体DNA 基因间隔区 多倍体起源 

分 类 号：Q789[生物学—分子生物学]