花生叶片离体培养花器官分化及其开花结果研究  

In Vitro Floral Organ Differentiation and Development of Peanut(Arachis hypogaea L.) Leaflets

作　　者：刘风珍[1] 万勇善[1] 王洪刚[1] 胡晓君[1] 

机构地区：[1]山东农业大学农学院作物生物学国家重点实验室,山东泰安271018

出　　处：《西北植物学报》2008年第6期1118-1121,共4页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家863计划项目(2006AA10A115);山东省中青年科学家科研奖励基金项目(2006BSB01049);国家公益性行业科研专项(Nyhyzx07-14)

摘　　要：以花生幼叶为外植体进行离体培养,研究BA浓度对花器官分化的影响并进一步观察试管内花器官的发育.结果表明:经MSB+1mg/LBA+0.5mg/LKIN+2mg/LIAA培养基诱导的愈伤组织,转接到附加1～3mg/LBA的MSB培养基上培养,均能直接诱导分化花器官,但2mg/LBA的诱导效率最高达21.13%;诱导分化的花器官转接到MSB培养基继续培养,部分花器官可以在试管内开花、受精、成针、结实.试验实现了以花生幼叶为外植体,在试管内完成诱导花芽、开花、受精、形成果针、子房膨大,直至形成荚果等过程,为离体条件下研究花生花器官分化、荚果及种子发育提供了技术体系和材料.The effects of BA concentrations on floral organ differentiation of leaflet-derived calluses of peanut were studied. The development of floral organ in test tube was observed. The results showed that： calluses generated from peanut leaflets in medium MSB＋1 mg/L BA＋0.5 mg/L KIN＋2 mg/L IAA,were sub cultured in media MSB supplemented with 0,1,2,3,4 and 5 mg/L BA,respectively. Floral organs were differentiated in media MSB supplemented with 1 -3 mg/L BA. The induction efficiency of floral organs in medium MSB＋2.0 mg/L I3A is 21.13% ,and is the highest. The types of regenerated floral organs included flower buds, petals and peduncles. Some of the regenerated flower buds have completed the breeding process from in vitro flowering,fertilization,and peg initiation up to pod setting. The technical system for floral organs regeneration from calluses induced from peanut leaflets and for normal development of the floral organs via in vitro were established.

关 键 词：花生 叶片离体培养 花器官分化 

分 类 号：Q813.13[生物学—生物工程]