胰腺癌中TFPI-2基因的表达及其意义  

Expression of TFPI-2 gene in human pancreatic carcinoma and its clinical significance

作　　者：汤志刚[1] 孙振阳[1] 胡何节[1] 许戈良[1] 陈炯[1] 李建生[1] 

机构地区：[1]安徽医科大学附属安徽省立医院普通外科,合肥230001

出　　处：《中华肝胆外科杂志》2008年第7期474-476,共3页Chinese Journal of Hepatobiliary Surgery

基　　金：本课题受安徽省自然科学基金（050430702）资助

摘　　要：目的 探讨人胰腺癌中TFPI2基因表达及其意义。方法 采用RTPCR及Westernblot技术，检测TFPI-2基因在50例胰腺癌、35例转移灶、8例正常胰腺组织中的表达，同时用Boyden小室及裸鼠皮下种植瘤模型检测胰腺癌细胞系Panc一1体内、体外侵袭及转移能力。结果 胰腺癌组织及转移灶中TFPI2mRNA及相应蛋白表达水平均低于正常胰腺组织（P〈o．05）。TFPI-2的表达水平与性别、年龄、肿瘤大小及组织学分级无关，而与临床分期、尤其是有无远处转移及神经侵犯有关。Ⅲ、Ⅳ期胰腺癌组织中TFPI-2表达水平低于I、Ⅱ期（P〈0．05）；无远处转移、无神经侵犯的胰腺癌组织中TFPI2表达水平高于有远处转移、有神经侵犯的胰腺癌组织（P〈0．05）。转染基因胰腺癌细胞系Panc-1-TFPI-2体外侵袭能力下降，无明显肌层浸润及肝、肺转移现象。结论 TFPI-2基因表达的降低与胰腺癌侵袭和转移有关，可能参与胰腺癌的侵袭和转移调控。Objective To investigate the expression of TFPI-2 in human pancreatic carcinoma and its clinical significance. Methods RT PCR and Western blot were used to detect the expression of TFPI 2 in 50 pancreatic carcinoma, 35 metastasis, 8 normal pancreatic tissue. Boyden chamber and subcutaneous neoplasm model was used to evaluate the effect of TFPI-2 on invasion and metastasis in vitro and in vivo. Results The expression of TFPI-2 mRNA and TFPI-2 protein in pancreatic carcino ma and metastasis was lower than that of normal pancreatic tissue （P〈0.05）. The expression of TF PI 2 had nothing to do with gender,age,size of the tumor and the grade in histology but was related to clinical stage,especially distant metastasis and invasion of nerves. The expression of TFPI 2 in pancre atic carcinoma in the clinical stage of Ⅲ, Ⅳ was lower than that in the clinical stage of I , Ⅱ （P〈 0. 05）. The expression of TFPI 2 in pancreatic carcinoma with distant metastasis and nerve invasion was lower than that of with no distant metastasis and nerves invasion （P〈0.05）. Boyden chamber as say showed that the invasion ability was lower in Panc-1-TFPI-2 group than that in Panc-l-V group and Panc 1 P group. In Panc 1 P and Panc-l-V group, the neoplasm invaded into muscular coat and metastasized to liver and lung tissue. But in Panc 1 TFPI 2 group,there was no infiltration of muscu- lar coat and metastasis in liver and lung. Conclusion The expression of TFPI-2 is relevant to invasion and metastasis of pancreatic carcinoma. TFPI-2 may play an important role in regulation of invasion and metastasis of pancreatic carcinoma.

关 键 词：胰腺肿瘤 肿瘤转移 组织因子途径抑制物2(TFPI-2) 

分 类 号：R686[医药卫生—骨科学]