丹参酮对肺缺血再灌注损伤的保护作用  

Immunoregulation of radix tanshinone on isolated-lung preservation

作　　者：王永剑[1] 舒平[1] 

机构地区：[1]北京大学深圳医院急诊科,广东省深圳518036

出　　处：《中国基层医药》2008年第5期705-706,共2页Chinese Journal of Primary Medicine and Pharmacy

基　　金：广东省深圳市科技局科研项目（JH200507131071A）

摘　　要：目的探讨丹参酮对肺再灌注损伤的保护作用。方法以离体兔肺模型为肺保护实验对象，以肺动脉灌洗再次肺动脉灌注为实验方法，以新西兰兔为实验动物，随机分为两组。一组用低钾右旋糖酐液行肺动脉灌洗和保存（对照组），另一组用丹参酮加LPD液组成实验组，用同样的方法灌洗和保存。保存18h后，观察病理形态并测量一氧化氮、超氧化物歧化酶及丙二醛的含量。结果光镜下两组肺泡、支气管及毛细血管结构完整，高倍视野下对照组较实验组肺泡上皮及毛细血管上皮细胞肿胀、肥大，少部分组织可见肺泡内有红细胞渗出、间隔增宽等。实验组肺组织中一氧化氮[（2．16±2．15）μmol／g]及超氧化物歧化酶[（37．03±18．01）NU／mg]含量高于对照组[（0．68±0．33）μmol／g、（17．63±11．04）NU／mg]（均P〈0．05），丙二醛含量[（0．47±0．20）μmol／mg]低于对照组[（0．86±0．44）μmol／mg]（P〈0．05）。结论丹参酮能清除氧自由基．有较强的抗氧化性，能较明显地减轻肺的再灌注损伤。Objective The study is to investigate the effect of tanshinone on lung reperfusion injury. Methods 24 New-Zealand rabbits were randomly divided into two groups. In one group,the lungs were perfused and preserved with LPD solution,the other with LPD ± tanshinone solution. The isolated lungs were preserved for 18 hours and then reperfused with rewarming whole blood. After reperfusing 30 minutes the lung samples were examined by pathology,wet/dry weight and measure of NO,SOD and MDA. Results In two groups,the reperfusion injury lungs were fresh,but had partial congestion, edema and ecchymoma under naked eye, under light microscopy the structures of the pulmonary vesiculars, branchia and capillaries were complete.but the epithelial cells of the vesicles and capillar- ies in the LPD group were more swelling and hypertrophy than that in LPD ± tanshinone group. The wet/dry weight ratio was significantly lower in LPD ± tanshinone group than in the LPD group. NO[ （2.16 ± 2.15 ）μmol/g] and SOD[ （37.03 ± 18.01 ） NU/mg] were higher in the LPD ± tanshinone group than in the LPD group[ （0.68 ± 0.33）μmol/g, （ 17.63 ± 11.04 ） NU/mg] （all P 〈 0.05 ）, MDA was lower in the LPD ± tanshinone group[ （0.47 ± 0.20 ） μmol/mg] than in the LPD group[（0.86±0.44）μmol/mg]（P〈0.05）.Conclusions Tanshinone can clean oxygen free radical and is of stronger antioxidation, results in alleriating of lung reperfusing injury. Tanshinone has positive effect on preservative quality ofthe reperfusion injury lung.

关 键 词：丹参酮 自由基 再灌注损伤 肺 兔 

分 类 号：R28[医药卫生—中药学]