缺铁乳铁蛋白体外抗HBsAg分泌的研究  

Study of inhibition effect on hepatitis B surface antigen of apolactoferrin in vitro

作　　者：诸晓强[1] 周海波[2] 李松涛[3] 黄桂荣[3] 刘宁[1] 

机构地区：[1]东北农业大学国家乳业工程技术研究中心,哈尔滨150030 [2]东北农业大学乳品科学教育部重点实验室食品学院,哈尔滨150030 [3]哈尔滨医科大学,哈尔滨150081

出　　处：《中国乳品工业》2008年第7期19-21,28,共4页China Dairy Industry

基　　金：黑龙江省普通高校骨干教师创新能力资助计划(1055G004)

摘　　要：研究缺铁乳铁蛋白体外抑制HBsAg作用,为乳铁蛋白应用于临床治疗乙肝病毒感染提供理论和实验依据。以天然HBV感染HepG2细胞为模型,通过应用ELISA法测定细胞上清中的HBsAg水平来检测缺铁乳铁蛋白抗HBsAg分泌效果,并用MTT法进行缺铁乳铁蛋白对HepG2细胞毒性研究。结果表明,缺铁乳铁蛋白对细胞的半数中毒剂量(TD50)为8.312 g/L,最大无毒剂量(TD0)为1.5 g/L;用乙肝病毒对HepG2细胞进行感染,分别加入不同浓度的缺铁乳铁蛋白,24,36,48 h测定HBsAg水平,各实验组蛋白对HBsAg分泌几乎没有抑制作用。当HepG2细胞感染乙肝病毒后,缺铁乳铁蛋白不能抑制HBsAg分泌,乳铁蛋白抗HBsAg分泌与其铁饱和度有关,但其机理有待进一步深入研究。To probe inhibition effect on hepatitis B surface antigen of apolactoferrin in vitro, so as to provide theoretical as well as experimental basis for the clinical management of HBV infection. Nature hepatitis B virus （HBV） was used to infect HepG2 cell as model, hepatitis B surface antigen was measured by ELISA to detect the effect of apolactoferrin on hepatitis B surface antigen secretion, and MTT test was used to examine cytotoxic effect of apolactoferrin. The maximum nontoxic dose（TD0） in HepG2 cell of apolactoferrin is 1.5 g/L. After HepG2 cell were infected with HBV, every Apolactoferrin test group had nearly no effect on inhibiting hepatitis B surface antigen to some extent. After HepG2 cell was infected with HBV, hepatitis B surface antigen could not be inhibited by Apolactoferrin, but the possible mechanism need to be lucubrated.

关 键 词：缺铁乳铁蛋白 体外 乙型肝炎表面抗原 HEPG2细胞系 ELISA 

分 类 号：Q936[生物学—微生物学]