异丙酚对全身高温所致大鼠认知障碍和海马神经元凋亡的影响  

Effects of propofol on whole body hyperthermia-induced cognitive handicap and hippocampus neurons apoptosis in rats

作　　者：卜祥梅[1] 赵鑫[1] 江淼[2] 王志刚[1] 

机构地区：[1]山东大学第二医院麻醉科,山东济南250012 [2]山东大学第二医院动物实验室

出　　处：《中国老年学杂志》2008年第14期1382-1384,共3页Chinese Journal of Gerontology

基　　金：山东省科学技术发展计划项目资助课题(2007GG20002014)

摘　　要：目的观察异丙酚对全身高温(whole body hyperthermia,WBH)导致的老年大鼠认知功能障碍和海马区神经元凋亡的影响。方法采用热辐射法建立大鼠WBH模型,用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测海马神经元凋亡,免疫组织化学法检测海马神经元天冬氨酸特异性半胱氨酸蛋白酶-3(caspase-3)蛋白的表达;用Morris水迷宫试验检测大鼠的认知功能。结果WBH过程中用异丙酚麻醉,能够减少海马神经元caspase-3蛋白的表达和海马神经元的凋亡,减轻大鼠的认知障碍。结论异丙酚能减少WBH引起的大鼠海马神经元凋亡,减轻WBH导致的大鼠认知功能障碍。Objective To investigate the effects of propofol on whole body hyperthermia （WBH） -induced cognitive handicap and hippocampus neurons apoptosis in rats. Methods WBH rat model was established by radiant-heat method to detect hippocampus neuron ap- optosis by TUNEL and caspases-3 expression by immunohistochemical method and cognition function by Morris water maze. Results Anes- thesia with propofol during WBH decreased caspase-3 protein expression and neuron apoptosis in hippocampus and alleviated cognitive handi- cap. Conclusions Propofol can decrease hippocampus neurons apoptosis and caspase-3 expression and alleviate cognitive handicap induced by WBH.

关 键 词：细胞凋亡 认知障碍 海马 水迷宫 全身高温 

分 类 号：R456.1[医药卫生—治疗学]