流动注射化学发光法测定法莫替丁  

FI-Chemiluminescence Determination of Famotidine

作　　者：何树华[1] 何德勇[2] 

机构地区：[1]长江师范学院化学系,重庆408003 [2]井冈山学院化学化工学院,井冈山343009

出　　处：《理化检验（化学分册）》2008年第7期650-652,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：重庆市教委科学研究项目(KJ051305)

摘　　要：在弱碱性的多聚磷酸钠介质中,二价铜离子、过氧化氢和染料罗丹明B混合可以产生较强的化学发光,法莫替丁对此发光有较强的抑制作用,据此并结合流动注射技术,提出一种直接测定法莫替丁的化学发光方法。其抑制发光强度与法莫替丁的质量浓度在5～1 000μg·L^(-1)范围内呈线性关系,检出限为2.7μg·L^(-1)(S/N=3),对300μg·L^(-1)法莫替丁平行测定11次,其相对标准偏差为2.1%。该方法已用于针剂和片剂中法莫替丁含量的测定,并测得方法的回收率在99.5%～102.8%之间,对方法的化学机理也作了简要探讨。In a weakly allkaline medium of sodium polyphosphate of pH 8. 68, chemiluminescence （CL）, produced by the reaction among cupric ion, hydrogen peroxide and rhodamine B was strongly inhibited by the drug, famotidine. By applying the flow injection technique, a method of determination of famotidine by CL was proposed. Magnitude of CL intensity inhibited was found to keep linear relationship with the concentration of famotidine in the range of 5. 0- 1 000 μg· L^-1 with a detection limit （as defined by IUPAC） of 2. 7 μg · L^-1. Precision of the method was tested at the concentration level of 300 μg· L^-1 for 11 determinations, giving value of RSD 2. 1%. The proposed method was applied to the analysis of famotidine injection and tablet samples, giving values of recovery in the range of 99. 5% to 102. 8%. The chemism of the CL-inhibition process was also discussed briefly.

关 键 词：化学发光 流动注射分析 法莫替丁 

分 类 号：O657.37[理学—分析化学]