高效液相色谱法测定水果和蔬菜中残留的多菌灵、吡虫啉和甲基托布津  

Determination of Residual Carbendazol,Imidacloprid and Thiophanate Methyl in Fruits and Vegetables by HPLC

作　　者：刘文杰[1] 万英[1] 庞新安[1] 张利莉[1] 

机构地区：[1]新疆塔里木大学生物技术研发中心新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,阿拉尔843300

出　　处：《理化检验（化学分册）》2008年第7期673-674,677,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：新疆生产建设兵团博士基金资助课题

摘　　要：建立了一个溶剂萃取后高效液相色谱法测定水果和蔬菜中残留农药多菌灵、吡虫啉和甲基托布津的方法。样品中残留农药用二氯甲烷与冰乙酸混合溶剂萃取,然后在Atlantis^B dC_(18)色谱柱上分离,用甲醇及0.1 mol·L^(-1)盐酸的混合溶液作梯度淋洗,洗出液中的农药在267 nm波长处紫外检测。方法对多菌灵、吡虫啉和甲基托布津的回收率分别为84%,83%,89%;相对偏差分别为10.68%,6.60%,9.08%。A method based on solvent extraction combined with HPLC for the determination of residual carbendazol, imidacloprid and thiophanate methyl in fruits and vegetables was proposed. The residual insecticides in sample were extracted with a mixed solvent of dichloromethane and acetic acid, and separated on an AtlanticsB dC18 column, Gradient elution with a mixed solution of methanol and 0. 1 mol · L^-1 HC1 was performed and the insecticides in the eluate were determined by UV-detection at 267 nm. Recoveries of the method were 84% for carbendazol, 83% for imidacloprid and 89% for thiophanate methyl, with relative deviation of 10. 68%, 6.60%, 9.08% respectively.

关 键 词：高效液相色谱法 农药残留 多菌灵 吡虫啉 甲基托布津 水果 蔬菜 

分 类 号：O657.7[理学—分析化学]