采用酶标凝集素检测特异的糖蛋白  

ASSAY OF SPECIFIC GLYCOPROTEIN BY ENZYMELINKED LECTIN

作　　者：周素芳[1] 吴耀生[1] 曾麒燕[1] 邓勇[1] 周德义[1] 

机构地区：[1]广西医科大学生物化学教研室

出　　处：《广西医科大学学报》1997年第4期58-60,共3页Journal of Guangxi Medical University

基　　金：国家自然科学基金

摘　　要：红桂木凝集素（ＡＬＬ）和木菠萝凝集素（Ｊａｃａｌｉｎ）经过碘酸钠法标记辣根过氧化物酶（ＨＲＰ）得到ＡＬＬ－ＨＲＰ和Ｊａｃａｌｉｎ－ＨＲＰ的酶标复合物。通过对１６种蛋白质进行检测，发现这两种凝集素均与胃蛋白酶、β－乳球蛋白结合（ＡＬＬ还可与牛血清白蛋白结合），其中与胃蛋白酶的结合最强。糖抑制试验表明半乳糖、棉子糖均可抑制两种凝集素对胃蛋白酶的作用。利用ＡＬＬ－ＨＲＰ酶联法检测１５例正常人胃液中的胃蛋白酶，酶含量为（１．２２５±０．７９５）ｍｇ／Ｌ。说明ＡＬＬ和Ｊａｃａｌｉｎ有许多相似的生物学特性，利用它们的这些特性可检测或分离纯化特异的糖蛋白。ALLHRP and JacalinHRP were prepared by method NaI O4,in which ALL and Jacalin were linked with HRPFrom the results of assaying 16 proteins,it was found that both ALL and Jacalin could combine with pepsin and βlactoglobulin.The affinity of lectin with pepsin was stronger than with βlactoglobulinCarbohydrate inhibitine test showed that galactose and raffinose could decrease the affinity of these two lectins with pepsin.Assaying by ALLHRP enzymelinked method,the pepsin content in gastric juice of 15 mormal subjects was found to be(1225±0795)μg/ml.

关 键 词：红桂木 木菠萝 酶标凝集素 糖蛋白 检测 

分 类 号：Q513.203[生物学—生物化学] Q592.9