Study on cellular internalization of poly(vinyldiaminotriazine)-based hydrogen bonding type non-viral transgene vector  

作　　者：YE GuiXiang CAO ZhiQiang LIN Lin CHEN DaYong LIU WenGuang 

机构地区：[1]School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University,Tianjin 300072, China

出　　处：《Chinese Science Bulletin》2008年第15期2307-2314,共8页

基　　金：the National Natural Science Foundation of China (Grant No. 30770587) ;Program for New Century Excellent Talents in University of China

摘　　要：Previously we successfully prepared poly(vinyldiaminotriazine)(PVDT)-based non-viral vectors which complexed plasmid DNA via hydrogen bonding with adenine-thymine base pairs. In this report, surface charges and complex sizes of this system were further examined. The results showed that PVDT-based polymer could cover surface charges of DNA resulting in slightly negative or neutral complexes. It was also found that the complex sizes were governed by two events: the aggregation induced by the insta-bility of neutral particles, and more compact complexes produced by PVDT-based polymers. In the study of cellular uptake, chlorpromazine and filipin Ⅲ were used to inhibit clathrin- and caveolae-me- diated endocytosis, respectively. We found that PVDT-based systems were transported into cells via a non-clathrin, non-caveolae mediated endocytosis. This special process was studied by temperature inhibition and kinetics assays. It was revealed that such a pathway was characterized by (i) a more en-ergy dependent process and (ii) a much slow transfection-effective internalization.Previously we successfully prepared poly（vinyldiaminotriazine）（PVDT）-based non-viral vectors which complexed piasmid DNA via hydrogen bonding with adenine-thymine base pairs, in this report, surface charges and complex sizes of this system were further examined. The results showed that PVDT-based polymer could cover surface charges of DNA resulting in slightly negative or neutral complexes. It was also found that the complex sizes were governed by two events： the aggregation induced by the instability of neutral particles, and more compact complexes produced by PVDT-based polymers. In the study of cellular uptake, chlorpromazine and filipin III were used to inhibit clathrin-and caveolae-mediated endocytosis, respectively. We found that PVDT-based systems were transported into cells via a non-clathrin, non-caveolae mediated endocytosis. This special process was studied by temperature inhibition and kinetics assays. It was revealed that such a pathway was characterized by （i） a more en- ergy dependent process and （ii） a much slow transfection-effective internalization.

关 键 词：聚乙烯 络合物 氢键 细胞提取 

分 类 号：O64[理学—物理化学]