保肝益胃合剂对四氯化碳致大鼠急性肝损伤的保护作用  

Protective effects of mixture for protecting liver and supplementing stomach (保肝益胃合剂) on rat acute liver damage induced by carbon tetrachloride

作　　者：姜树民[1] 单静喜[1] 任江[1] 刘冰[1] 王宝柱[1] 孙科峰[1] 付勇强[1] 王晖[1] 王英[1] 尤春来[1] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中国中西医结合急救杂志》2008年第4期219-221,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家中医药管理局中医;中西医结合急诊临床基地建设项目

摘　　要：目的观察保肝益胃合剂对四氯化碳(CCI_4)所致大鼠急性肝损伤的保护作用。方法腹腔注射CCl_42 ml/kg建立大鼠急性肝损伤模型。分别用保肝益胃合剂(30 g·kg^(-1)·d^(-1))、多烯磷脂酰胆碱胶囊(易善复,180 mg·kg^(-1)·d^(-1))、甘草酸二铵胶囊(甘利欣,30 mg·kg^(-1)·d^(-1))灌胃,联合治疗组则用保肝益胃合剂(30 g·kg^(-1)·d^(-1))+易善复(180 mg·kg^(-1)·d^(-1))灌胃。检测各组大鼠血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)活性,同时观察肝脏组织病理学变化以及肝细胞坏死评分和大鼠死亡率。结果保肝益胃合剂组ALT、AST活性和肝细胞坏死评分[(1.168±1.066)kU/L、(1.845±2.212)kU/L、(0.56±0.53)分]均显著低于模型组[(4.982±3.502)kU/L、(7.030±3.616)kU/L、(1.38±0.92)分,P均<0.01]。正常对照组大鼠无死亡;保肝益胃合剂组和保肝益胃合剂+易善复组大鼠死亡率(分别为37.5%、25.0%)均较模型组(60.0%)明显降低,以联用组为最低(P<0.05)。结论保肝益胃合剂对CCl_4所致大鼠急性肝损伤有明显的保护作用。Objective To observe the protective effects of a mixture for protecting liver and supplementing stomach （保肝益胃合剂） on rat acute liver damage induced by carbon tetrachloride （CC14）. Methods The model of rat acute liver damage was established by injection of CC14 2 ml/kg into the abdominal cavity. The rat models were treated respectively by the mixture for protecting liver and supplementing stomach 30 g · kg^-1· d^-1, the polyene phosphatide acid radical choline capsule [Yi Shanfu （易善复）, 180 mg· kg^-1· d^-1], the glycyrrhizic acid diaminogen capsule [Gan Lixin （甘利欣）, 30 mg · kg^-1· d^-1） infused into the stomach. The activities of serum alanine transaminase （ALT） and aspartate transaminase （AST） were detected. In the mean time, the liver pathological changes were observed, the degree of liver cell necrosis was evaluated, and the rat mortality was noted in various groups of treatment. Results The values of ALT, AST and the score of liver cell necrosis in the group treated with the mixture for protecting liver and supplementing stomach [（1. 168 ± 1. 066） kU/L, （1. 845 ± 2. 212） kU/L, （0.56 ± 0.53） score） were significantly lower than those in the model group [（4.982±3.502） kU/L, （7.030±3.616） kU/L, （1.38±0.92） scores）, and all the differences being statistically significant （all P〈0.01）. In the normal control group, there was no death of rat. The mortality rates in the group of the mixture for protecting liver and supplementing stomach and the group of the mixture for protecting liver and supplementing stomach plus Yi Shanfu （37.5%, 25.0%） were significantly lower than the mortality in the model group （60.0%, both P〈0.05）. Conclusion The mixture for protecting liver and supplementing stomach has marked protective effects on acute liver damage induced by CC14 in rats.

关 键 词：保肝益胃合剂 四氯化碳 肝损伤 急性 细胞坏死评分 大鼠 

分 类 号：R285.5[医药卫生—中药学] R256.4[医药卫生—中医学]