消痞方对糖尿病胃轻瘫大鼠胃c-kit基因表达变化的影响  

Effect of Xiaopi prescription (消痞方) on mRNA expression of c-kit in the rat with diabetic gastroparesis

作　　者：穆标[1] 曲竹秋[1] 刘之武[1] 崔海沫[1] 秦怡男[1] 熊湘明[1] 阳泽彬[1] 

机构地区：[1]天津市公安医院消化科,天津300040

出　　处：《中国中西医结合急救杂志》2008年第4期229-232,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

摘　　要：目的探讨Cajal间质细胞(ICC)在糖尿病大鼠胃轻瘫发病机制中的作用以及中药消痞方的防治疗效。方法50只健康雄性SD大鼠被随机均分为正常对照组、糖尿病模型组及消痞方低、中、高剂量治疗组。静脉注射四氧嘧啶建立大鼠糖尿病模型;正常对照组只注射等量生理盐水。低、中和高剂量组分别用中药消痞方5、10和20 g·kg^(-1)·d^(-1)灌胃给药;正常对照组、模型组仅给予等量生理盐水灌胃。用营养性半固体米糊法测定胃排空率;免疫组化法、原位杂交法分别对c-kit阳性的ICC及c-kit mRNA表达进行定量分析。结果①胃排空率:模型组较正常对照组明显降低(P<0.05);消痞方中、高剂量组与正常对照组比较差异无统计学意义(P均>0.05),但较模型组及消痞方低剂量组均明显增高(P均<0.05);消痞方低剂量组与模型组比较差异无统计学意义(P>0.05);②c-kit免疫组化:c-kit阳性细胞呈棕黄色,膜着色,主要分布于环形肌与纵行肌间的肌间神经丛周围,并围绕神经节细胞形成类似"鞘样"结构。各组c-kit阳性细胞含量的定量分析结果是:模型组较正常对照组明显降低(P<0.05);消痞方中、高剂量组与正常对照组比较差异无统计学意义(P均>0.05),但较模型组明显升高(P均<0.05);消痞方低剂量组与模型组比较差异无统计学意义(P>0.05),均显著低于正常对照组和消痞方中、高剂量组(P均<0.05);③c-kit mRNA表达量:c-kit mRNA阳性物质呈棕黄色,胞质着色,主要分布于环形肌与纵行肌间的肌间神经丛周围,并围绕神经节细胞形成类似"鞘样"结构。c-kit mRNA的表达量为:模型组较正常对照组明显降低(P<0.05);消痞方中、高剂量组与正常对照组比较差异无统计学意义(P均>0.05),但较模型组明显升高(P均<0.05);低剂量组与模型组比较差异无统计学意义(P>0.05),但显著低于正常对照组和消痞方中、高剂量组(P均<0.05)。结论①糖尿病胃轻�Objective To investigate the possible role of interstitial cell of Cajal （ICC）in the pathogenesis of diabetic gastroparesis and the protective effect of ＂Xiaopi prescription （消痞方）＂. Methods Fifty healthy male Spregue-Dawley （SD） rats were randomly divided into 5 groups （each n = 10）： normal, model, and 3 Xiaopi prescription groups： low, middle and high dosages. Diabetes was induced by intravenous injection of alloxan, and equal amount of normal saline was intravenously injected in the normal group. Gastric lavage method was used to administer the traditional Chinese medicine decoction of Xiaopi prescription in corresponding amount （5, 10 and 20 g · kg^-1· d^-1） in respective low, middle and high dosage groups. In the normal control group and diabetic model group, only equal amount of normal saline was administered into the stomach. Gastric emptying rate was measured by method of nutritious semisolid paste; c-kit positive cells of ICC were quantitatively measured with immunohistochemistry assay and computer image analysis system; c-klt mRNA positive cells were quantitatively measured with in situ hybridization and computer image analysis system. Results ①astric emptying rate： The rate was significantly lower in the model group than that in the normal control group （P〈0.05）; in the middle and high dosage groups of Xiaopi prescription, the rates were not significantly different from that in the normal control group （all P〉0.05）, but higher than those in the model group and the low dosage group （all P〈0.05）, respectively; and there was no marked difference between low dosage group and the model group （P〉0.05）. ②c-kit immmunohistochemistry： c-kit positive cell presented yellow in color, and its membrane was stained yellow, this kind of cells primarily were distributed around the neural plexus in the inter-space between the circular and longitudinal muscular fibers, and around the ganglionic cells forming ＂sheath-like＂ structure. The results of num

关 键 词：糖尿病 CAJAL间质细胞 胃动力 消痞方 

分 类 号：R285.5[医药卫生—中药学] R587.1[医药卫生—中医学]