Bcl-2及FasL蛋白的表达与子宫内膜异位症发生发展的关系  

Relationship between Bcl-2/FasL gene and pathogenesis of endometriosis

作　　者：钟蕙芳[1] 林婧[1] 

机构地区：[1]上海交通大学附属国际和平妇幼保健院,上海200030

出　　处：《中国实验诊断学》2008年第7期898-900,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的研究Bcl-2(B细胞淋巴瘤/白血病基因-2蛋白)和FasL(体内Fas蛋白的天然配体)基因与子宫内膜异位症(EMS)之间的关系。方法采用免疫组化法(SP法)来检测Bcl-2与FasL在正常子宫内膜(19例),异位内膜(33例)及在位内膜(11例)中的表达。结果①正常内膜组:Bcl-2与FasL主要表达于细胞浆及细胞膜上,Bcl-2在增生期的表达显著高于分泌期(P<0.05),FasL在增生期的表达显著低于分泌期(P<0.05),即Bcl-2及FasL在正常子宫内膜细胞中的表达有明显周期性。②异位内膜组:Bcl-2及FasL的表达同样位于细胞浆或细胞膜,分泌期的表达与增生期相似(P>0.05),因此在异位子宫内膜中的Bcl-2与FasL呈持续表达且丧失周期性,且二者在异位内膜中的表达显著高于正常内膜(P<0.0001)。结论以上结果提示具有抗凋亡活性的子宫内膜细胞逆流至盆腹腔后,因其高水平Bcl-2及FasL表达,得以逃避机体免疫清除系统的影响而在异位存活、种植,为子宫内膜异位症的发生创造了先决条件。Objective To research whether the endometriosis is associated with Bel-2 and FasL gene. Methods Immunohisto- chemistry method （streptavidin - peroxidase method） was used to detect the expression of Bel-2 and FasL gene in 19 specimens of normal endometrium tissue, 33 specimens of endometriosis tissue and eutopie endometrium from patients with endometriosis （11 eases） .Results The control group：the expression of Bel-2 and FasL predominated in the glandular cytoplasm and/ or on the plasma membrane .The expressions of Bel-2 in proliferative phase was higher than that in secretory phase signifieantly （ P 〈 0.05） .The expressions of FasL in proliferative phase was less than that in secretory phase signifieantly （ P 〈 0.05）. So the expressions of Bel-2 and FasL obviously cyclic changes throughtout the menstrual cycle in nomal endometrium tissue.2. The endometriosis group： The expressions of Bel-2 and FasL also predominated in the glandular cytoplasm and/or on the plasma membrane. Both Bel-2 and FasL expression in proliferative phase was the same as secretory phase （ P 〉 0.05）. So Bel-2 and FasL expression in adenomyosis had no cyclic change and their staining had remained in the whole menstrual cycle. The expressions of Bel-2 and FasL in endometriosis was higher than that in normal endometrium signifieandy（ P 〈 0.000 1 ）. Conclusion It is suggested that the retrograding of these active antiapoptotie endometrial cells may play a key role in the pathogenesis of endometriosis.

关 键 词：子宫内膜异位症 BEL-2 FASL 免疫组织化学 细胞凋亡 

分 类 号：R711.71[医药卫生—妇产科学]