基质金属蛋白酶9在多囊卵巢综合征患者血清中表达增高  

Raised serum levels of matrix metalloproteinase-9 in women with polycystic ovary syndrome

作　　者：刘彬[1] 张雅娟[1] 张洪秀[1] 吕荟明[1] 夏蕾[1] 关咏梅[1] 

机构地区：[1]哈尔滨医科大学附属第一医院生殖医学科,哈尔滨150001

出　　处：《中国优生与遗传杂志》2008年第8期22-23,28,共3页Chinese Journal of Birth Health & Heredity

基　　金：黑龙江省青年科学技术专项资金资助项目(QC05C34)

摘　　要：目的近来有研究提示,基质金属蛋白酶(matrix metalloproteinases,MMPs)可能通过调节卵巢内细胞外基质成分的降解和重建,参与多囊卵巢综合征(polycystic ovary syndrome,PCOS)的病理发生。本研究旨在探讨MMP-9在PCOS发病中可能的作用机制。方法采用病例-对照研究,ELISA法检测PCOS患者和正常对照组血清MMP-9及其抑制因子TIMP-1(tissue inhibitor of metalloproteinase-1,TIMP-1),胰岛素样生长因子I(insulin-like growth factor-I,IGF-I)和胰岛素样生长因子结合蛋白1(insulin-like growth factor binding protein-1,IGFBP-1)的蛋白表达水平。结果P-COS患者血清中MMP-9浓度和MMP-9/TIMP-1比值明显高于对照组。在PCOS组,血清IGFBP-1水平与MMP-9呈负相关。结论MMP-9的高表达可能通过调节IGFs的生物利用度参与PCOS的病理发生,其意义和解释需要进一步研究。Objective： It has been suggested in recent studies that matrix metalloproteinases （MMPs） may be implicated in the pathogenesis of polycystic ovary syndrome （PCOS） through regulating ovarian tissue remodeling. This study was aimed to investigate the possible role of MMPs in the pathophysiology of PCOS. Methods ： Serum levels of MMP - 9, tissue inhibitor of metalloproteinase - 1 （ TIMP - 1 ） , insulin - like growth factor - I （ IGF - I） and insulin - like growth factor binding protein - 1 （ IGFBP - 1 ） were meas- ured in 40 patients with PCOS and 40 healthy women with regular menstruation, matched for age and body mass index. Correlation between IGFBP - 1 and other parameters in PCOS group was analyzed by Pearson,s linear correlations and multiple variable models. Re- sults ： The serum concentrations of MMP - 9 and the ratio of MMP - 9/TIMP - 1 were significantly higher in PCOS women than in controis. Serum levels of IGFBP - 1 were markedly lower in PCOS group. There was a negative correlation between serum IGFBP - 1 and MMP- 9 in women with PCOS. Conclusion： Our results raise the possibility that MMPs may be implicated in the pathophysiology of P- COS either by regulating ovarian tissue remodeling or indirectly by facilitating IGF - I bioavailability through proteolysis of IGFBP - 1.

关 键 词：基质金属蛋白酶9 胰岛素样生长因子结合蛋白1 多囊卵巢综合征 细胞外基质 

分 类 号：R711.75[医药卫生—妇产科学]