猪水疱病重组蛋白P1的豚鼠免疫试验  

Immunological analysis of the swine vesicular disease virus (SVDV) recombinant P1 protein in Guinea pigs

作　　者：田宏[1] 吴锦艳[1] 尚佑军[1] 郑海学[1] 孙世琪[1] 刘湘涛[1] 谢庆阁[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室,兰州730046

出　　处：《中国免疫学杂志》2008年第7期626-629,共4页Chinese Journal of Immunology

基　　金：国家863高技术研究发展计划(2003AA241110)资助项目

摘　　要：目的:研究重组猪水疱病病毒(SVDV)P1蛋白的免疫活性。方法:将自制的猪水疱病抗原经佐剂乳化免疫豚鼠,并应用T淋巴细胞增殖试验、阻断ELISA及微量细胞中和试验检测了所制备的免疫原免疫豚鼠后在体液免疫和细胞免疫方面进行了全面的评价。结果:前期制备的抗原免疫豚鼠后具有良好的免疫原性。结论:对该亚单位疫苗可做进一步的开发应用。Objective-To investigate the immtmogeneicity of a subunit vaccine of capsid protein precursor（P1） of swine vesicular diseas（SVD）. Methods： In this study, the guinea pigs were immunized with the home-made antigen, T-lymphocyte proliferation response, blocking EIJSA and micro-neutralization assay were used to detect the effect of the immunized responses in guinea pigs. Results： The results indicated that a retroviral-based vaccine carrying the capsid protein precursor（P1 ） of SVD was able to elicit strong SVDV-spocific humoral immune responses in guinea pigs. Conclusion：It encourages further work towards the development of a vaccine against SVDV infection.

关 键 词：猪水疱病病毒 亚单位疫苗 豚鼠 免疫试验 

分 类 号：S852.659.6[农业科学—基础兽医学]