高温正相色谱/反相色谱(HTNPLC/RPLC)二维联用系统的构建与应用  

Construction and Application of Two-dimensional Liquid Chromatography(High Temperature NPLC/RPLC) System

作　　者：田宏哲[1] 徐静[2] 关亚风[2] 

机构地区：[1]沈阳农业大学分析测试中心,辽宁沈阳110161 [2]中国科学院大连化学物理研究所仪器分析研究室,辽宁大连116023

出　　处：《分析测试学报》2008年第7期691-696,共6页Journal of Instrumental Analysis

摘　　要：为解决含水正相色谱与反相色谱联用过程中的谱带展宽问题,以高温正相色谱(HTNPLC)为第一维,室温反相色谱(RPLC)为第二维,分别建立了切割模式的二维液相色谱(HTNPLC-μRPLC)和全二维液相色谱(HTNPLC×RPLC)系统。样品首先在第一维(CN色谱柱)进行正相分离,第一维洗脱产物选择性地或者交替存储在十通阀上的定量环中,然后切换十通阀将定量环内存储的样品组分转移到第二维(C18色谱柱)进行反相分离。该系统降低了第一维切割组分在第二维柱头的扩散,提高了分离度以及分离效率。采用多环芳烃混合物和天然植物甘草提取液对该二维液相色谱系统进行了评价。To overcome the spreading of peak width problem and to increase the peak capacity and separation efficiency of two-dimensional liquid chromatography system,a comprehensive two-dimensional liquid chromatography（HTNPLC×RPLC） system and a heart-cutting two-dimensional liquid chromatography（HTNPLC-μRPLC） system were constructed by using high temperature NPLC as the first dimensional（1^st-D） mode and RPLC as the second dimensional（2^nd-D） separation mode.The sample was first separated on the 1^st-D CN column and the primary eluent alternatively（in HTNPLC×RPLC） or selectively（in HTNPLC-μRPLC） stored in the sampling-loop system was then transferred to 2^nd-D C18 column for further separation.The advantages of these systems were that the degree and efficiency of the separation were greatly improved.The evaluations of the performances of the systems were performed by analyzing several polycyclic aromatic hydrocarbons and glycyrrhiza uralensis extract.

关 键 词：二维液相色谱 高温正相色谱/反相色谱 定量环-阀切换接口 中药 甘草 

分 类 号：O657.72[理学—分析化学]