硫酸阿米卡星的毛细管电泳法快速测定  

Rapid Determination of Amikacin Sulfate in Amikacin Sulfate Injection by Capillary Electrophoresis

作　　者：翟海云[1] 路新卫[2] 吴燕红[1] 陈缵光[3] 

机构地区：[1]广东药学院中药开发研究所,广东广州510006 [2]南方医科大学药学院,广东广州510515 [3]中山大学药学院,广东广州510089

出　　处：《分析测试学报》2008年第7期769-771,共3页Journal of Instrumental Analysis

基　　金：广东省科学计划资助项目(2004B10401027);广东药学院博士启动基金资助项目(43551006)

摘　　要：建立了毛细管电泳高频电导法快速测定注射液中硫酸阿米卡星的新方法。考察了缓冲溶液、有机溶剂添加剂以及分离电压和进样条件等因素对分离检测的影响。采用5.0 mmol/L乳酸-30%（体积分数）乙醇缓冲液体系为运行电泳介质,分离电压20.0 kV,在最佳实验条件下,5.5 min内即可实现硫酸阿米卡星的分离检测,硫酸阿米卡星的线性范围为5.00-150 mg/L,检出限为1.5 mg/L。A rapid method for the determination of amikacin sulfate in amikacin sulfate injection solution by capillary electrophoresis with high frequency conductivity detection was described.The influences of the key factors such as running buffer,organic additive,separation voltage and inject time were discussed in detail.Amikacin sulfate could be separated and detected within 5.5 min under the following separation conditions：5.0 mmol/L lactic acid-30.0%（by volume）C2H5OH as running buffer,20.0 kV separation voltage.Amikacin sulfate showed good linear response in the range of 5.00-150 mg/L,and the limit of detection was 1.5 mg/L.

关 键 词：毛细管电泳 高频电导检测 硫酸阿米卡星 

分 类 号：O657.8[理学—分析化学] R978.1[理学—化学]