何首乌中磷脂类化合物的微波辅助萃取/高效液相色谱法测定  

Determination of Phospholipids in Polygonum Multiflorum Thunb by Microwave Assisted Extraction/High Performance Liquid Chromatography

作　　者：万益群[1] 吴世芳[2] 

机构地区：[1]南昌大学食品科学教育部重点实验室,江西南昌330047 [2]南昌大学分析测试中心,江西南昌330047

出　　处：《分析测试学报》2008年第7期782-784,787,共4页Journal of Instrumental Analysis

基　　金：长江学者和创新团队发展计划资助项目(IRT0540);南昌大学食品科学教育部重点实验室开发基金资助项目(200408)

摘　　要：利用微波辅助萃取(MAE)技术对何首乌中磷脂类化合物的萃取条件进行研究,并通过液相色谱法分离测定。通过对MAE影响因素的考察,确定萃取溶剂为氯仿-甲醇(体积比1∶2)的混合溶剂,固液比为1∶20,萃取时间15 min,温度45℃。在HPLC中,采用Nucleosil 100-5硅胶色谱柱(250 mm×4.6 mm i.d.,5μm),甲醇为流动相,流速0.8 mL/min,进样量15μL,蒸发光散射检测器。Phospholipids in Polygonum multiflorum Thunb were extracted using microwave assisted extraction（MAE）and then analyzed by high performance liquid chromatography（HPLC） in this study.In MAE,chloroform-methanol（1 ∶2,by volume） was selected as the extraction solvent with the solid to liquid ratio of 1 ∶20,extraction time of 15 min and the temperature of 45 ℃.Phospholipids were separated on a Nuclesil 100-5 column（250 mm×4.6 mm i.d.,5 μm）with methanol as the mobile phase at a flow rate of 0.8 mL/min and detected by a evaporative light-scattering detector.The method was effective and suitable for quality control of Polygonum multiflorum Thunb and related preparations.

关 键 词：微波辅助萃取 高效液相色谱法 何首乌 磷脂 

分 类 号：O657.72[理学—分析化学] Q545[理学—化学]