三七总皂苷对脑缺血再灌注大鼠BDNF表达的影响  

Effect of panax notoginseng saponinsa on the expression of BDNF in rats with cerebral ischemia reperfusion

作　　者：张永全[1] 莫国焕[1] 陈明 曾祥发[1] 陆晖[1] 

机构地区：[1]广西中医学院附属瑞康医院神经内科,广西南宁530011 [2]梧州制药集团股份有限公司,广西梧州543002

出　　处：《中成药》2008年第7期958-961,共4页Chinese Traditional Patent Medicine

摘　　要：目的:三七总皂苷对大鼠大脑中动脉脑缺血/再灌注(MCA-CI/RP)保护作用及机制。方法:参考Longa线栓法建立大鼠MCA-CI/RP动物模型。根据Longa 5分法进行神经功能评分,应用TTC染色法测定脑梗死体积,TUNEL技术原位标记凋亡细胞,免疫组化法检测脑源性神经营养因子(brain-derived neurotrophic factor BDNF)的表达。结果:三七总皂苷可以缩小MCA-CI/RP大鼠脑梗死体积、神经细胞凋亡及梗死后神经功能缺损程度,并可促进神经功能恢复和增加缺血脑组织内源性BDNF的表达。结论:三七总皂苷可能通过增加缺血脑组织内源性BDNF的表达而产生神经保护作用,大剂量三七总皂苷组优于小剂量组。AIM： To study the protective effect and mechanism of panax notoginseng saponinsa （PNS） for dealing with rats with middle cerebral artery ischemia reperfusion （CI/RP）. METHODS： To establish the rat middle cerebral artery ischemia/reperfusion model with suture occlusion technique and record the neurologic impairment evaluation score on the basis of Zea Longa method, measured the infarct volume, and the others were detected neuron apoptosis by TUNEL technology and endogenous brain-derived neurotropic factor （BDNF） expression by immunohistochemistry technology. RESULTS： The administration of PNS to rat decreased neurologic impairment, reduced infarct volume, inhibited the neuron apoptosis, and increased endogenous BDNF expression after 24 hours CI/RP. CONCLUSION： Increasing endogenous BDNF expression after CI/RP might be the mechanisms of PNS for achieving neuroprotections.

关 键 词：三七总皂苷 川芎嗪 脑梗死 细胞凋亡 脑源性神经营养因子 

分 类 号：R285.6[医药卫生—中药学]