大鼠血清中复方清开灵药物原型的HPLC-MS^n和HPLC-TOF-MS分析  

Analysis of medicine components from Compound Qingkailing in rat serum

作　　者：庞丽琼[1] 刘清飞[2] 梁琼麟[1] 冉小蓉[1] 王义明[1] 罗国安[1] 

机构地区：[1]清华大学分析中心,北京100084 [2]清华大学医学院,北京100084

出　　处：《中成药》2008年第7期1024-1027,共4页Chinese Traditional Patent Medicine

基　　金：科技部十一五支撑项目“中药复方作用机理和中药复方配伍评价研究”;清华大学基础研究基金(JCqn2005038)

摘　　要：目的:建立大鼠血清中复方清开灵(牛黄、珍珠母、板蓝根、水牛角、黄芩、栀子、金银花)药物原型成分的HPLC-MSn和HPLC-TOF-MS联用分析方法。方法:取静脉注射清开灵的大鼠血样,甲醇沉淀蛋白。对血样进行TOF-MS分析可以给出精确的分子量和分子式,而电喷雾离子阱多级质谱扫描,可以给出目标质荷比的多级碎片,两者结合对原型成分进行准确的鉴定。结果:识别和鉴定了大鼠血清中复方清开灵的四种主要原型成分栀子苷,黄芩苷,汉黄芩苷和胆酸。结论:该方法准确快速,是检测体内样品中复方中药原型成分的高效分析方法。AIM： To establish methods for identifying the antetype of Compound Qingkailing （Calculus bovis, Concha margaritifera, Radix isatidis, Cornu bubali） in rat serum by HPLC-MS^n and HPLC-TOF-MS. METH- ODS： Addition of methanol to serum after Qingkailing given intravenously to rats was used to precipitate protein. The HPLC-TOF-MS provided the exact molecular weight and the HPLC-ESI-MS^n provided the m/z of multilevel fragment. The medicine antetypes were identified by combining the two methods. RESULTS： Four main medicine antetypes in rat serum, such as geniposide, baicalein, wogonoside and cholic acid, were identified. CONCLUSION ： It is a rapid and exact method that can be used to identify other complicated traditional Chinese medicine in vivo.

关 键 词：清开灵注射液 药物原型 HPLC-MS^N HPLC-TOF-MS 

分 类 号：R927.1[医药卫生—药学]