壳聚糖对甲硝唑凝胶体外促透作用研究  

Study of Chitosan on the Transdermal Permeation Enhancing Effect by Metronidazole Gels in vitro

作　　者：刘新云[1] 余红梅[2] 何秀丽[1] 叶立红[1] 肖淼生[1] 

机构地区：[1]郧阳医学院附属太和医院药学部,湖北十堰442000 [2]郧阳医学院附属太和医院内分泌科,湖北十堰442000

出　　处：《医药导报》2008年第8期886-888,共3页Herald of Medicine

基　　金：湖北省十堰市科技攻关项目(基金编号:2006051D)

摘　　要：目的考察壳聚糖(CS)对甲硝唑凝胶体外透皮速率的影响。方法将1.0%CS作为吸收促进剂用于甲硝唑凝胶中,以泊洛沙姆p407为凝胶基质,以含2%氮酮的甲硝唑凝胶作为阳性对照,不含任何促渗剂的甲硝唑凝胶作为阴性对照,采用改良Franz扩散池进行大鼠体外皮肤渗透实验,反相高效液相色谱法(RP-HPLC)法测定接受液中甲硝唑含量,计算累积透过量Q,得出Q-t回归方程及稳态渗透速率J。结果1.0%CS组和氮酮组J分别为2.841,2.874μg.(cm2)-1.h-1,两者差异无显著性(P>0.05),而与阴性组相比均差异有显著性(均P<0.05),其透皮吸收行为符合一级方程。结论CS对甲硝唑凝胶的体外透皮吸收有较好的促进作用,值得进一步研究。Objective To study the effect of chitosan （CS） on cutaneous absorption of metronidazole gel in vitro. Methods 1.0% CS was used as penetration enhancer in metronidazole gels, which contained 2% of azone as positive control, and that with no penetration enhancer as the negative control. The rats skin was treated as cutaneous barrier, the cumulative penetrationamount （Q） of Azone group and CS groups, respectively was determined by RP-HPLC assay to calculate the stable penetration rate J. Results J of metronidazole gels including 2% Azone and 1.0% CS was 2. 841 and 2.874 μg·（cm^2）1·h^-1 , respectively, which showed no significant difference （P 〉 0.05） between each other. However, both displayed significant difference with that in metronidazole gels without any enhancer （P 〈 0.05 ）. Condusion 1.0% CS could enhance cutaneous absorption of metronidazole, which deserves further research.

关 键 词：壳聚糖 甲硝唑 透皮吸收促进剂 凝胶 体外 

分 类 号：R944[医药卫生—药剂学] R978[医药卫生—药学]