大气混合污染物致大鼠肺氧化损伤作用  

Effects of air mixed pollutants on lung damage in rats

作　　者：赵肃[1] 王任群[1] 王灿[1] 金焕荣[1] 肖纯凌[1] 

机构地区：[1]沈阳医学院公共卫生学院职业与环境卫生学教研室,沈阳110034

出　　处：《中国公共卫生》2008年第8期981-982,共2页Chinese Journal of Public Health

基　　金：沈阳市科技局(1032043-1-0101)

摘　　要：目的探讨大气混合污染物对大鼠肺损伤的作用。方法48只Wistar大鼠随机分为4组,即对照组和实验组(实际、中、高剂量组),进行可吸入颗粒物(PM10)及混合气体(SO2、NO2、CO)染毒。染尘采用一次性气管注入法,混合气体染毒采用静式吸入法,每天2 h,连续30 d。测定血清及支气管肺泡灌洗液(BALF)中相关生化酶的活性和脂质过氧化指标水平。结果实验组BALF中的乳酸脱氢酶(LDH)活性(4.03,2.98,3.63 U/ml)及总蛋白水平(1.14,0.71,0.98 g/L)均显著高于对照组(1.91 U/ml,0.37 g/L),差异有统计学意义。高剂量丙二醛(MDA)平均水平(2.10 mmol/ml)明显高于对照组(0.95 mmol/ml,P<0.05);实验组超氧化物歧化酶(SOD)活性(18.32,25.72,16.82 U/ml)均明显低于对照组(40.88 U/ml,P<0.01);血清中各指标水平变化不明显。结论大气混合污染物可引起肺的氧化损伤,并导致大鼠肺细胞膜的通透性增强。Objective To explore the damage mechanism of lung in rats exposed to air mixed pollutants. Methods 48 Wistar rats were randomly divided into control group, actual -, middle - and high - dose groups. Treated rats were exposed to PM10 by intratracheal instillation and then were exposed to SO2, NO2 and CO by static inhalation 2 hours per day for 30 days. The activities and contents of enzymes in the brochoalveolar lavage fluid （BALF） and the serum were determined. Results The activities of LDH （4.03, 2.98, 3.63 U/ml） and the levels of proteins （1.14, 0.71,0.98 g/L） in BALF in rats of treated groups were higher than that of control group （1.91 U/ml, 0.37 g/L）. The level of MDA in BALF in rats of highdose group averaged 2.10 mmol/ml （control group 0.95 mmol/ml） and the activities of SOD in BALF in rats of treated rats averaged 18.32, 25.72, 16.82 Nu/ml （control group 40.88 Nu/ml）. There were significant differences compared with control group. The levels of all indexes in serum were not significant between different groups. Conclusion Air mixed pollutants may result in oxidative damage in pulmonary tissue and may increase the membrane permeability of pulmonary cells in rats.

关 键 词：大气混合污染物 大鼠 支气管肺泡灌洗液 氧化损伤 

分 类 号：R563[医药卫生—呼吸系统]