多西紫杉醇纳米结构脂质载体中主药含量及包封率测定  

Determination of the Content and Entrapment Efficiency of Decetaxel in Drug Loaded Nano-structured Lipid Carrier

作　　者：李翔[1] 陈颖 王雅晖 贺小玲[1] 赵春晖[1] 王东凯[1] 

机构地区：[1]沈阳药科大学,沈阳市110016 [2]辽宁省药材有限责任公司,沈阳市110001

出　　处：《中国药房》2008年第22期1743-1745,共3页China Pharmacy

摘　　要：目的:建立测定多西紫杉醇(DTM)纳米结构脂质载体(NLC)中主药含量及包封率的方法。方法:采用高效液相色谱法测定药物含量,色谱柱为DiamonsilTMODS,流动相为乙腈-水(55:45),流速为1.0mL.min-1,检测波长为228nm;分别采用超速离心法及超滤法分离DTX-NLC中游离药物以测定包封率。结果:DTM检测浓度的线性范围为0.024～2.4μg.mL-1(r=0.9996),平均回收率为99.29%(RSD=0.37%);2种方法所测药物的平均包封率均>96.5%。结论:本方法简便、准确、可靠,灵敏度高,可用于该制剂的质量控制。OBJECTIVE： To establish a RP - HPLC method for determination of the content and entrapment efficiency of docetaxel （DTX） in drug loaded nano-structured lipid carrier （NLC） . METHODS： The separation was performed with a Diamonsi^TM ODS column, and the mobile phase consisted of acetonitrile-water （55 ： 45） with a flow rate of 1.0mL·min^-1 and UV detection wavelength at 228 nm. Ultracentrifugation and ultrafiltration methods were employed to isolate free decetaxel for the entrapment efficiency determination. RESULTS： Docetaxel had a good linear relation over the range of 0.024-- 2.4 μg · mL^-1（r=0.999 6）, the average recovery was 99.29% （RSD = 0.37%） ; The average entrapment efficiency by 2 kinds of methods were more than 96.5%. CONCLUSION： This method is simple, accurate, sensitive and applicable for quality control of this preparation.

关 键 词：多西紫杉醇 纳米结构脂质载体 舍量测定 包封率 高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学]