高效液相色谱法测定盐酸坦洛新中S异构体的含量  

Determination of S-Isomer in Tamsulosin Hydrochloride by HPLC

作　　者：吴琼诗[1] 何雷萍[2] 杜迎翔[2] 狄斌[3] 

机构地区：[1]海南省人民医院药学部,海南海口570311 [2]中国药科大学分析化学教研室,江苏南京210009 [3]中国药科大学药物分析教研室,江苏南京210009

出　　处：《中国药业》2008年第15期27-28,共2页China Pharmaceuticals

摘　　要：目的建立测定盐酸坦洛新中S异构体含量的高效液相色谱法。方法采用Daicel Chiralcel OD-RH柱(250mm×4.6mm,5μm)为色谱柱,流动相为乙腈-pH值为4.0的0.8mol/L高氯酸钠溶液(25:75),流速0.5mL/min,检测波长224nm,柱温30℃。结果盐酸坦洛新R异构体与S异构体的分离度达2.0,S异构体的线性范围为0.4995～7.992μg/mL(r=0.9998),平均回收率为98.7%(n=9)。结论高效液相色谱法操作简便,灵敏精确,可用于盐酸坦洛新中S异构体的含量测定。Objective To establish a HPLC method for the determination of the S- isomer in tamsulosin hydrochloride. Methods The column was Diacel Chiralcel OD-RH （250 mm×4.6mm, 5 μm）.The mobile phase was acetonitrile-0. 8 mol/L sodium perchlorate （25：75, V/V, pH =4. 0） with the flow rate of 0. 5 mL/min. The wavelength was 224 nm. The column temperature was 30 ℃. Results The resolution of the enantiomers of tamsulosin hydrochloride was 2. 0. The linear range of the S-isomer of tamsulosin hydrochloride was 0. 499 5-7. 992 g/mL （r=0. 999 8）. The average recovery was 98.7% （n =9）. Conclusion The method is simple and accurate. It can be used for the determination of S- isomer in tamsulosin hydrochloride.

关 键 词：高效液相色谱法 盐酸坦洛新 S异构体 含量测定 

分 类 号：TQ460.72[医药卫生—药物分析学] R927.11[化学工程—制药化工]