RAPD技术分析积雪草的分子标记特征  

Molecular Characters of Centella asiatica Found with RAPD Technology

作　　者：阮元[1] 莫荣浩[1] 李敏[1] 黄浏清[1] 罗育[1] 李雄英[1] 周娟[1] 吴耀生[1] 

机构地区：[1]广西医科大学,广西南宁530021

出　　处：《中药材》2008年第7期970-973,共4页Journal of Chinese Medicinal Materials

基　　金：广西科学基金(桂科基0575065;桂科基0731065);广西区教育厅硕士研究生科研创新项目(2006105981001M02)

摘　　要：目的:采用RAPD技术,对积雪草DNA分子标记进行研究。方法:以不同来源的积雪草为样本提取基因组DNA,通过优化PCR反应体系,选择适合的随机引物,扩增分析积雪草的特征条带。结果:经优化反应体系扩增,获得不同来源及不同生长环境的积雪草特异的种群条带,具有高度稳定的遗传特征。积雪草的种群特征带与绞股蓝、烟草、乌蔹莓等其他不同科属的植物有明显的区别。结论:所建立的RAPD分析方法扩增不同来源的积雪草显示高度的均一性,可用于积雪草真品和混淆品的鉴别。Objective：To analyze the DNA molecular characters of Centella asiatica with RAPD technology.Methods：With the genomic DNA as templates extracted from various source of Centella asiatica samples,optimized RAPD PCR reaction systems had been used.The random promers had been screened to amplify the specific molecular fragments of Centella asiatica.Results：The specific genetic bands of Centella asiatica species from various habitats were established which were highly stable and repeatable and obviously different from those of other families, genuses of plants such as Gynostemma pentaphylum,Tobacco,Cayratia japonica.Conclusion：The developed method of RAPD analysis for the genetic character bands of Centella asiatica could be applied to identify real Centella asatica from its spurious breed plants.The genetic character bands of Centella asiatica amplified with the RAPD method show high homogeneous in several samples from different habitats.

关 键 词：积雪草 RAPD DNA分子标记 鉴别 

分 类 号：R282.5[医药卫生—中药学]