利用稻瘟霉分生孢子追踪分离黑乳海参活性成分  

Screening of Bioactive Constituents from Sea Cucumber Holothuria nobilis Using Conidia of Pyricularia oryzae

作　　者：张佳佳[1] 戴静波[1] 陈莉莉 丁萍月 巫军[2] 

机构地区：[1]浙江医药高等专科学校药物分析教研室,浙江宁波315200 [2]宁波拜奥生物医药科技,浙江宁波315201

出　　处：《中药材》2008年第7期1001-1003,共3页Journal of Chinese Medicinal Materials

基　　金：国家高技术研究发展计划(863计划)(2001,AA624100);上海市科委攻关项目(02DZ19101);宁波市自然科学基金项目(2770A610083)

摘　　要：目的:研究黑乳海参中海参皂苷类活性成分。方法:以稻瘟霉分生孢子活性追踪结果为指导,运用多种层析分离技术和光谱学方法对黑乳海参中的海参皂苷性成分进行分离并鉴定其结构。结果:分离并鉴定了12个化合物,包括7个新的海参皂苷(nobiliside D-J)。所有皂苷类化合物均对稻瘟霉有活性,且对P388小鼠白血病和A549人肺癌细胞株具有显著或较强的细胞毒作用。结论:通过该研究为研制新的抗肿瘤药物提供了先导化合物,为充分开发利用我国的海洋生物资源提供了物质基础和科学依据。Objective：To study the bioactive triterpene glycosides from sea cucumber Holothuria nobilis.Methods：Guided by Pyricularia.oryzae bioassay method,the glycosides compounds were separated by multi-chrmatography,and their structures were elucidated by chemical and spectral analysis.Results：Twelve triterpene glycosides were obtained and their structures were identified,including seven novel saponins.All saponins showed activities on P.oryzae,and most of them exhibited cytotoxicity on P388 and A549 significantly.Conclusion：Our researches provided valuable leads for pursuing new anticancer drugs,and established a homebase for further dovelopment of H.nobilis which is widely distributed in South China Sea.

关 键 词：稻瘟霉 黑乳海参 皂苷 细胞毒 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]