溃结灵对溃疡性结肠炎大鼠结肠粘膜IKK-α蛋白表达的作用  

Effect of Kuijieling Decoction on Proteins Expression of IKK-α in Colonic Mucosa of Ulcerative Colitis Model Rats

作　　者：杜群[1] 李红[2] 王汝俊[1] 李燕舞[1] 巫燕莉[1] 王建华[1] 

机构地区：[1]广州中医药大学脾胃研究所,广东广州510405 [2]广州中医药大学中药学院,广东广州510006

出　　处：《中药材》2008年第7期1016-1018,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(30400613)

摘　　要：目的:观察溃结灵对溃疡性结肠炎(UC)大鼠结肠粘膜IKK-α蛋白表达的作用,对其作用机制进行初步探讨。方法:采用三硝基苯磺酸(TNBS)法制作UC大鼠模型,大鼠随机分为六组:正常对照组、模型对照组、溃结灵低、中、高剂量组、SASP阳性对照组。治疗10 d后处死大鼠取结肠组织,采取免疫组化(IHC)法检测IKK-α的阳性细胞表达率。结果:模型组结肠粘膜IKK-α蛋白阳性细胞表达率明显高于正常对照组(P<0.01),溃结灵中剂量组结肠粘膜IKK-α蛋白阳性细胞表达率明显低于模型组(P<0.05),溃结灵高剂量组和SASP阳性对照组结肠粘膜IKK-α蛋白阳性细胞表达率明显低于模型组(P<0.01)。结论:溃结灵使TNBS法UC大鼠模型结肠粘膜IKK-α蛋白阳性细胞表达率明显降低,这可能是其治疗UC作用的机理之一。Objective：To explore the changes of protein expression of IKK-α as well as the effects of Kuijieling Decoction（KD） on colonic mucosa of ulcerative colitis（UC） model rats.Methods：UC model rats were induced by TNBS.The rats were randomly divided into six groups：normal control（NC） group,model control（MC） group,Kuijieling low dose（KLD）,middle dose（KMD） group,high dose（KHD） group and SASP group.After 10-days′ treatment the rats were killed to get their colonic tissues.The positive rate of IKK-α expression was detected by immunohistochemical（IHC）.Results：The positive rate of IKKα in MC group was significantly higher than that in NC group（P〈0.01）.The positive rate of IKK-α in KMD group was significantly lower than that in MC group （P〈0.05）.The positive rate of IKK-α in KHD and SASP group were significantly lower than that in MC group（P〈0.01）.Conclusion：IKK-α may be involved in the pathogenesis of UC,and KD can inhibit positive rate of IKK-α in colonic mucosa of UC model rats induced by TNBS.The inhibitory effects of KD on UC may be associated with this.

关 键 词：溃结灵 溃疡性结肠炎 大鼠模型 IKK-α 

分 类 号：R285.5[医药卫生—中药学]