HPLC法测定胃安颗粒中延胡索乙素的含量  

Determination of tetrahydropalmatine in Weian Granules by HPLC

作　　者：王玲[1] 

机构地区：[1]山东中医药大学附属医院,山东济南250011

出　　处：《齐鲁药事》2008年第7期401-402,共2页qilu pharmaceutical affairs

摘　　要：目的建立胃安颗粒中延胡索乙素的含量测定方法.方法采用高效液相色谱法测定胃安颗粒中延胡索乙素的含量.色谱柱:Hypersil C18柱(4.6mm×250mm,5μm);流动相:甲醇-0.1%磷酸溶液(55∶45);流速:1mL·min-1;柱温:室温;检测波长:280nm.结果延胡索乙素在0.193～0.965μg范围内,进样量与峰面积呈良好的线性关系(r=0.9992),平均回收率为96.59%,RSD=1.99%.结论本法准确、专属性强、重现性好,可作为胃安颗粒中延胡索乙素的含量测定方法.OBJECTIVE To establish an HPLC method for determination of tetrahydropalmatine in Weian Granules. METHODS Hypersil C18 column （4. 6mm × 250mm, 5um）was used. The mobile phase was methanol -0. 1% phosphoric acid solution （55 ： 45）. The flow rate was 1.0mL · min^-1. The detection wavelength was 280nm. RESULTS Tetrahydropalmatine had a good linear relationship between 0. 193-0. 965ug,average recovery was 96. 59%, RED was 1.99%. CONCLUSION This method was rapid, simple,and accurate, can be used for the determination of tetrahvdrooalmatine in Weian Granules.

关 键 词：胃安颗粒 延胡索乙素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]