Isolation and Identification of Burkholderia glumae from Symptomless Rice Seeds  

作　　者：ZHU Bo LOU Miao-miao HUAI Yan XIE Guan-lin Luo Jin-yan Xu Li-hui 

机构地区：[1]State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310029, China

出　　处：《Rice science》2008年第2期145-149,共5页水稻科学（英文版）

基　　金：the National Natural Science Foundation of China (Grant No. 30671397)

摘　　要：A survey on isolation and detection of the casual organism of bacterial grain rot of rice was conducted during 1997-2006. In 2006, six pathogenic bacterial strains were isolated from two symptomless seed samples of rice （Oryza sativa L.） originally produced in Hainan Province and then planted in Zhejiang Province, China. They were identified as Burkholderia glumae which is the causal organism of bacterial grain rot of rice by physiological characteristics, colony morphology, pathogenicity test, Biolog, fatty acid methyl ester （FAME） analysis and RAPD-PCR compared with the four standard reference strains. It is confirmed that there is the infection of B. glumae in so-called ＇health looking seeds＇.A survey on isolation and detection of the casual organism of bacterial grain rot of rice was conducted during 1997-2006. In 2006, six pathogenic bacterial strains were isolated from two symptomless seed samples of rice （Oryza sativa L.） originally produced in Hainan Province and then planted in Zhejiang Province, China. They were identified as Burkholderia glumae which is the causal organism of bacterial grain rot of rice by physiological characteristics, colony morphology, pathogenicity test, Biolog, fatty acid methyl ester （FAME） analysis and RAPD-PCR compared with the four standard reference strains. It is confirmed that there is the infection of B. glumae in so-called ＇health looking seeds＇.

关 键 词：Burkholderia glumae ISOLATION molecular identification PATHOGENICITY bacterial grain rot rice seed 

分 类 号：S511[农业科学—作物学]