复方胆通片中大黄素的含量测定  

Determination of Emodin in Compound Dantong Tablet with HPLC Method

作　　者：刘伟玲[1] 

机构地区：[1]娄底市中心医院,湖南娄底417000

出　　处：《中医药导报》2008年第7期93-94,共2页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:建立复方胆通片中大黄素的含量测定方法。方法:色谱柱:kromasil C18柱;流动相:甲醇-0.1%磷酸溶液(85:15);检测波长:254 nm;流速:0.8 ml.min-1。结果:大黄素线性范围为2.63～26.3μg.ml-1,r=0.9997,回收率为98.08%,RSD%为1.33%。结论:本方法简便、可靠、重现性较好,能起到控制复方胆通片中大黄素含量的作用。Objective： To establish HPLC method for the detetrmination of emodin in Compound Dantong Tablet. Methods： The chromatographic conditions consisted of Hypersil Cms chromato bar with mobile phase of methanol-0.1% phosphoric acid（85：15） at the detection wavelength of 254 nm, the flow rate was 0.8 mL/min. Results： The linear relationship was good when emodin was at the range of 2.63-26.31μg.ml^-1 （r=0.9997）, the average recovery was 98.08%, RSD%=1.33%（n=6）. Conclusion： The method is simple, reliable, good reproducibility, can be used to control emodin in Compound Dantong Tablet.

关 键 词：复方胆通片 大黄素 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]