金龟子绿僵菌菌株生长环境变量的优化  

Optimization of environmental factors for the growth of Metarhizium anisopliae var. anisopliae strains MA4 and MAlm1

作　　者：杨腊英[1] 甘露[2] 刘丽[2] 文明富[2] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南省热带农业有害生物检测监控重点实验室,海南儋州571737 [2]华南热带农业大学农学院,海南儋州571737

出　　处：《生态学杂志》2008年第8期1322-1326,共5页Chinese Journal of Ecology

基　　金：国家科技基础条件平台工作资助项目(2005DKA21201-18);中央级公益性科研院所基本科研业务费专项(2007HZS1J007);国家科技支撑计划资助项目(2007BAD48B02)

摘　　要：金龟子绿僵菌(Metarhizium anisopliae var.anisopliae)是一种广谱的昆虫病原真菌,提高菌株的生长速率及产孢量具有重要的意义。分别对2株金龟子绿僵菌菌株MA4与MAlm1的菌丝生长及次代产孢具有影响的培养温度、培养基初始pH、装液比、光照及微量元素等环境因素进行了测定。经过优化得到2菌株最佳培养条件分别为:MA4菌株在28℃、pH7、装液比为75ml/250ml、加入微量元素Mn全光照培养时生长最好、次代产孢量最高;MAlm1菌株在28℃、pH9、装液比为75ml/250ml、加入微量元素Cu、黑暗培养时生长最好、次代产孢量最高。Metarhizium anisopliae vat. anisopliae is a kind of broad spectrum entomopathogenic fungi, and thus, to increase its growth rate and sporification is of important significance. In this study, the environmental factors affecting hypha growth and second generation sporulation of two M. anisopliae vat. anisopliae strains MA4 and MAlml, such as culture temperature, initial pH of culture medium, entire volume of liquid, illumination, and microelements, were determined. The results showed that the optimal growth and sporification conditions for MA4 strain were 28 ℃, pH 7, 75 ml/250 ml installment, 24 h light/clay with Mn addition; while those for MAlml strain were 28 ℃ , pH 9, 75 ml/250 ml installment, 0 h light/clay, and Cu addition. Key words： Metarhizium anisopliae var. anisopliae; environmental factors; hypha biomass; sec- ond generation sporulation.

关 键 词：金龟子绿僵菌 环境因子 菌丝生长量 次代产孢量 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]