脐血有核细胞输注治疗慢性肝衰竭的初步临床观察  

Human umbilical cord blood nucleated cell therapy in patients with chronic severe viral hepatitis

作　　者：唐晓鹏[1] 王文龙[1] 张旻[1] 曾妍[1] 田沂[1] 

机构地区：[1]中南大学肝病研究所湘雅第二医院感染科,湖南长沙410011

出　　处：《中国输血杂志》2008年第6期420-423,共4页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金资助课题(39870651);湖南省自然科学基金资助课题(04JJ6048)

摘　　要：目的探讨人脐血有核细胞移植(HUCBNCT)治疗慢性重型肝炎肝衰竭的临床治疗效果,以及HUCBNCT对重型肝炎患者肝组织内胆管增生及肝卵圆细胞增生的影响。方法将90名慢性重型肝炎患者随机分为2组,分别予以同血型成人血浆(对照组)或HUCBNCT(治疗组)治疗,2组中分别有部分患者行肝穿刺病理检查及肝组织细胞角蛋白19(CK19)、CD34、白蛋白和甲胎蛋白(AFP)检测。结果HUCBNCT组患者肝细胞坏死较轻,肝组织CK19阳性率高于对照组;部分非典型胆管增生细胞同时表达CK19和白蛋白,在形态上和细胞表型上符合肝卵圆细胞特征。结论HUCBNCT对于慢性重型肝炎具有一定的疗效,在改善肝脏功能方面优于同血型成人血浆,其促进慢性重型肝炎患者肝组织内非典型胆管细胞增生及肝卵圆细胞增生可能是其作用机制之一。Objective To investigate the therapeutic effect of hmnan umbilical cord blood nucleated cell therapy （HUCBNCr） in patients with chronic severe viral hepatitis, and the effect of HUCBNCT on ductular proliferation and hepatic oval cell proliferation. Methods A total of 90 patients with chronic severe viral hepatitis were divided into two groups randomly, and the two groups were treated with ad^t plasma transfusion （control group）, and HUCBNCT （treatment group）, respectively. The therapeutic effect in all patients was evaluated by determination of liver function. Some patients had liver biopsy and CK19, CIY34, albumin and AFP of the biopsy tissues were detected. Results Liver necrosis in treatment group was milder than that of control group, and the positive rate of CK19 staining in treatment group was higher than that of control group. Co-expression of CK19 and albumin were observed in some atypical ductular proliferation cells, which were like the oval cells in shape and immunohistochemical characteristics. Conclusion HUCBNCT, which can promote liver ductular and oval cell proliferation, may have promising therapeutic effect on patients with chronic severe viral hepatitis.

关 键 词：有核细胞 移植 肝炎 病毒性 人 脐带血 

分 类 号：R458.8[医药卫生—治疗学] R512.6[医药卫生—临床医学]