RU486对人早孕绒毛中纤维蛋白溶酶原激活与抑制系统的影响  

Influence of RU486 on the activating and inhibiting system of plasminogen in human early pregnancy villi

作　　者：陈贵安[1,2] 张彤[1,2] 朱艳[1,2] 冯强 刘以训 

机构地区：[1]北京医科大学第三医院 [2]中国科学院动物研究所计划生育生殖生物学重点实验室

出　　处：《现代妇产科进展》1997年第4期307-311,316,共6页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金;计划生育生殖生物学国家重点实验室基金

摘　　要：目的：探讨ＲＵ４８６对离体培养人早孕绒毛滋养细胞分泌ＰＡｓ及ＰＡＩ┐１、ＰＡＩ┐２的影响，比较药物流产与正常绒毛中ｔＰＡ、ｕＰＡ、ＰＡＩ┐１及ｕＰＡ┐Ｒ表达的差异。方法：应用纤维蛋白琼脂糖铺盖及反向铺盖技术分别检测细胞培养液中ＰＡ与ＰＡＩ┐１的活性，采用琼脂糖指示胶直接点样法测定ＰＡＩ┐２活性。分别应用免疫组织化学与原位杂交技术检测绒毛组织细胞中ｔＰＡ、ｕＰＡ、ＰＡＩ┐１与ｕＰＡ┐Ｒ的抗原及ｍＲＮＡ的存在与分布。结果：受ＲＵ４８６刺激，离体培养绒毛滋养细胞分泌的ＰＡ┐ＰＡＩ复合物的水解活性增加，而ＰＡＩ┐１与ＰＡＩ┐２的活性则受抑制。口服米非司酮药流绒毛滋养细胞中ＰＡＩ┐１及ｕＰＡ抗原含量低，而ｕＰＡ┐Ｒ的ｍＲＮＡ含量显著增加。结论：ＲＵ４８６增加早孕绒毛中纤溶活性，从而使细胞外基质蛋白水解作用加强，绒毛的固着性可能因此松动。Objective:To investigate the effects of RU486(mifepristone) on release of PAs and PAI1,PAI2 in cultured trophoblastic cells,and on expression of tPA,uPA,PAI1,uPAR in human early placental villi.Methods:SDSPAGE followed by fibrin overlaid and reverse overlaid techniques were used to determine PAs and PAI1 activities respectively in media for culture of trophoblastic cells.PAI2 activities from media were measured by PAI indicating agarose gel directely.The immunohistochemical and in situ hybridization methods were used to detect the antigens and mRNAs of tPA,uPA,PAI1 and uPA-R in early villi from women requesting termination of pregnancy of 6￣8w by curettage or drugabortion with RU486.Results:In vitro cultured trophoblastic cells secreted PAPAI complex and PAI1 and PAI2.RU486 increased fibrinolytic activity of PAPAI complexes and decreased activities of PAI1 and PAI2.Cytoand syncytiotrophoblastic cells in early villi contained antigens and mRNAs of tPA,uPA,PAI1 and uPAR.The antigen contents of PAI1 and uPA were lower in both trophoblastic cells from villi treated by RU486 than that in the normal one.While RU486 highly increased the mRNA of uPAR in the both cells.Conclusions:RU486 increases the activity of PAPAI complex,but decreases the activities of PAI1 and PAI2 in cultured trophoblastic cells in vitro,RU486 reduces PAI1,but increses mRNA of uPAR in both trophoblastic cells of villi in vivo.These effects of RU486 can activate the fibrinolytic processes in extracellular proteins to induce contraearlygestation.

关 键 词：米非司酮 中断妊娠 早孕绒毛 RU486 PA-PAI系统 

分 类 号：R979.22[医药卫生—药品] R169.42[医药卫生—药学]