多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫小鼠后脾细胞因子的动态观察  

Dynamic observation on cytokines of splenocytes in mice by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis

作　　者：李文桂[1] 王鸿[1] 朱佑明[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《中国病原生物学杂志》2008年第7期502-505,共4页Journal of Pathogen Biology

基　　金：国家自然科学基金资助项目(No.30671835、No.30500423和No.30200239);教育部重点项目(No.205131);重庆市教委科研基金项目(No.KJ050313);重庆市科委科研基金项目(No.03-43-8);重庆市卫生局科研基金项目(No.03-2-099)

摘　　要：目的动态观察多房棘球绦虫混合重组BCG-EmⅡ／3和BCG-Em14-3-3疫苗免疫小鼠后脾细胞因子的变化。方法将混合疫苗采用鼻腔内接种免疫BALB／c鼠，在免疫后0、2、4、6、8和10周各剖杀4只小鼠，取脾，分离脾细胞，用EmAg或ConA刺激培养，收集脾细胞培养上清液，测定IL-2、IFN-7、TNF-α和IL-4水平。试验设有PBS对照。结果疫苗接种组小鼠脾细胞IL-2、IFN-γ、TNF-α和IL-4分别在免疫后2～10周、4～8周、6～10周和4～6周升高，分别在免疫后6、6、8和4周达最高水平，含量分别为10．0pg／ml、（75．0±6．6）pg／ml、（245．0±83．0）pg／ml和（187．5±16．5）pg／ml。结论多房棘球绦虫混合重组BCG-EmII／3和BCG-Em14—3—3疫苗在免疫早期（2～8周）即可诱导小鼠产生TH1和TH2混合型免疫反应。Objective To dynamically observe changes of cytokines of splenocytes in mice by immunization with mix recombinant BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus rnultilocularis （Em）. Methods BALB/c mice were intranasally vaccinated by the mixed vaccine and killed to get spleen on 0, 2, 4, 6, 8 and 10 weeks post-immunization, respectively, splenocytes were separated to culture by stimulation with EmAg or ConA, their supernatants were gathered to measure level of IL-2, IFN-γ, TNF-α and II.-4 by ELISA Kits, PBS served as control. Results In the groups of immunization, levels of IL-2, IFN-γ, TNF-α and IL-4 increased obviously on 2--10 weeks, 4--8 weeks, 6-10 weeks and 4- 6 weeks, respectively, reached the highest level on 6, 6, 8 and 4 weeks, respectively, their contents were 10.0 pg/ml, （75.0±6.6） pg/ml, （245.0±83.0） pg/ml and （187.5± 16.5） pg/ml, respectively. Conclusion TH1 and TH2 mixed response was induced in mice by mix recombinant BCG-Em Ⅱ/3 and BCG-Eml4-3-3 vaccine on early immunization（2-8 weeks）.

关 键 词：多房棘球绦虫 重组BCG-EmⅡ/3疫苗 重组BCG-EM14-3-3疫苗 细胞因子 

分 类 号：R383.33[医药卫生—医学寄生虫学]