弓形虫感染对孕鼠及胎鼠染色体的影响  

Effect of Toxoplasma gondii infection on chromosome aberration in pregnant and fetal mice

作　　者：刘利[1] 李淑红[1] 崔黎明[2] 刘冰[3] 

机构地区：[1]吉林大学白求恩医学院病原生物学教研室,吉林长春130021 [2]吉林大学白求恩医学院病原免疫细胞遗传实验中心 [3]吉林大学白求恩医学院遗传学教研室

出　　处：《中国病原生物学杂志》2008年第7期520-522,共3页Journal of Pathogen Biology

基　　金：吉林省科技厅资助项目(No.19980300);长春市科技局资助项目(No.20037)

摘　　要：目的研究弓形虫感染对孕鼠及胎鼠染色体的影响,探讨弓形虫的致畸机制。方法通过姊妹染色单体互换(SCE)试验和微核(MN)试验,检测妊娠期感染弓形虫的小鼠骨髓细胞及胎鼠肝细胞的SCE和MN发生率。试验设阳性对照组和阴性对照。试验结果进行统计学分析(双侧t检验)。结果孕4d及孕11d感染弓形虫,孕鼠骨髓细胞SCE发生率实验组为(3.39±0.94)/细胞和(3.33±0.90)/细胞,阴性对照组为(3.89±0.97)/细胞,差异无统计学意义(P>0.05);胎鼠肝细胞SCE发生率实验组为(3.06±0.69)/细胞和(3.09±0.68)/细胞,对照组为(2.71±0.91)/细胞,差异无统计学意义(P>0.05)。孕鼠骨髓细胞MN发生率实验组为(3.00±0.53)‰和(2.75±0.71)‰,对照组为(2.50±0.89)‰,差异无统计学意义(P>0.05);胎鼠肝MN发生率实验组为(4.25±0.64)‰和(4.00±0.76)‰,对照组为(3.50±0.71)‰,差异无统计学意义(P>0.05)。结论妊娠期感染弓形虫,对孕鼠及胎鼠染色体无明显影响。Objective To investigate the mechanism of chromosome aberranon in pregnant and fetal mice induced by Toxoplasrna gondii. Methods The observation of both bone marrow cells of pregnant mice and liver of fetal mice carried out using sister ehromatid exchange （SCE） test and micronuleus （MN） test when the mice were infected with T, gondii. The experimental results were analysed by t test, Result Infected by T, gondii were at 4 d and 11 d. the frequency of SCE of maternal bone marrow cells in experimental group were （3, 39±0, 94） /cell and （3.33±0, 90）/cell. the differences were not obvious between experimental groups and negative eontral group （3.89± 0, 97）/cell（P〉0.05）, No significance of SCE in hepatocytes of foetal mice were observed between experimental （3.06±0.69） and （3. 09±0.68）/ cell and control group （2. 71±0. 91）/cell（P〉0.05）. The frequency of MN in bone marrow cells of pregnant mice were （3.00±0.53） and （2, 75±0, 71）‰ in experimental group, there was little difference between experimental group and control group （2.50±0, 89）‰（P〉0.05）. No significance was found about frequency of MN in liver of fetal mice between experimental （4. 25±0. 64）‰ and （4. 00±0.76）‰ and control group （3.50±0.71）‰（P〉0. 05） too, Conclu- sion Toxoplasrna gondii may not induce chromosome aberration in pregnant and fetal mice.

关 键 词：弓形虫 姊妹染色单体互换 微核 

分 类 号：R382.5[医药卫生—医学寄生虫学]