HBeAg阴性血清中乙型肝炎病毒基本核心启动子和前C区变异研究  

Study on the mutations of basal core promoter and precore gene of hepatitis B virus genome in HBeAg negative sera

作　　者：郭龙华[1] 董长垣[2] 黄宪章[1] 庄俊华[1] 林莉[1] 周强[1] 

机构地区：[1]广东省中医院二沙岛分院检验科,广东广州510105 [2]武汉大学医学院,湖北武汉430071

出　　处：《检验医学》2008年第4期333-337,共5页Laboratory Medicine

摘　　要：目的了解本地区乙型肝炎病毒(HBV)流行株基本核心启动子(BCP)和前C(PreC)基因的变异热点,为临床提供诊疗信息。方法通过设计3条特异性引物进行半巢式聚合酶链反应(PCR)扩增患者血清中HBV BCP和PreC基因序列,回收和纯化PCR产物,用引物P1、P2直接进行正反测序,然后进行比对分析。结果BCP变异主要集中在TATA样盒(TATA-like box)中的TA1、TA2、TA3区,而TA4极为保守,TA1-TA4未见插入或缺失突变,PreC变异主要集中在nt1896位G→A。另外,发现1例新奇的插入突变。结论BCP和PreC变异可使HBeAg阴转。Objective To investigate the mutations of the basal core promoter（BCP） region and precore gene in the HBeAg negative sera of patients with hepatitis B virus（HBV） infection in Guangzhou and provide information for diagnosis and treatment of the disease. Methods The BCP region and precore gene were amplified by heminested polymerase chain reaction （PCR） with three specific primers, and the reclaimed and purified PCR products were bi-directly sequenced by primers P1 and P2, and the sequences were analysed. Results The point mutations of BCP region always occurred in TA1, TA2 and TA3 in TATA-like boxes. There were no deletion or insertion mutations in TATA-like boxes. There was a hot point mutation in nucleotide position 1896G→A in precore gene. A novel insertion mutation in DR1 was found. Conclusions The mutations in BCP region and preeore gene could cause HBeAg seroeonversion.

关 键 词：乙型肝炎病毒 变异 基本核心启动子 前C基因 半巢式PCR 

分 类 号：Q503[生物学—生物化学]