应用Tn5-1uxAB-sacB基因盒对根瘤菌巨型质粒进行定向标记、缺失或消除  

USE TN5-LUXAB-SACB GENES CASSETTE FOR IABELLING、DELETION OR CURING THE MEGA-PLASMIDS OF RHIZOBIA

作　　者：莫才清[1] 郭先武[1] 周俊初[1] 李阜棣[1] 

机构地区：[1]华中农业大学生命科学技术学院,武汉430070

出　　处：《微生物学杂志》1997年第3期6-9,共4页Journal of Microbiology

基　　金：国家"863"资助;国家自然科学基金

摘　　要：pHNC3, a suicide plasmid encoding luxAB and scaB genes in Tn5,can be transfered into Rhizobium through tri-parental mating and thenthe Rhizobium is labelled by luxAB. Since the levansucrasc activitycoded by sacB gene can cause the sucrose sensitivity on Gram-negativebacteria,the sacB gene allowed direct selection for the deletion or curingof plasmide, because only strains which no longer contain an activesacB gene are able to grow on media containing sucrose. This negativeselecting model can be used for direct labelling the mega-plasmid ofRhizobium,or even getting Rhizobium deriyatives partial deleted or curedtheir mega-plasmids.pHNC3, a suicide plasmid encoding luxAB and scaB genes in Tn5,can be transfered into Rhizobium through tri-parental mating and thenthe Rhizobium is labelled by luxAB. Since the levansucrasc activitycoded by sacB gene can cause the sucrose sensitivity on Gram-negativebacteria,the sacB gene allowed direct selection for the deletion or curingof plasmide, because only strains which no longer contain an activesacB gene are able to grow on media containing sucrose. This negativeselecting model can be used for direct labelling the mega-plasmid ofRhizobium,or even getting Rhizobium deriyatives partial deleted or curedtheir mega-plasmids.

关 键 词：根瘤菌 巨型质粒 定向标 Tn5-1uxAB-scaB 基因盒 

分 类 号：Q939.114[生物学—微生物学]