四溴双酚-A和五溴酚对红鲫甲状腺激素和脱碘酶的影响  

Effects of tetrabromobisphenol-A and pentabromophenol on thyroid hormones and deiodinases of goldfish,Carassius auratus

作　　者：瞿璟琰[1] 施华宏[1] 刘青坡[1] 沈江帆[2] 

机构地区：[1]华东师范大学资源与环境学院环境科学系上海市城市化过程与生态恢复重点实验室,上海200062 [2]上海市普陀区中心医院核医学科,上海200062

出　　处：《环境科学学报》2008年第8期1625-1630,共6页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No20507007);长江水环境教育部重点实验室开放基金(NoYRWEF06003);上海市城市化过程与生态恢复重点实验室开放基金(NoUPER0606)~~

摘　　要：将红鲫(Carassius auratus)分别暴露于0.5mg·L-1四溴双酚-A(TBBPA)和0.1mg·L-1五溴酚(PBP)中28d,采用放射性免疫分析法和化学发光酶联免疫分析法检测红鲫的血清甲状腺激素水平和肝脏脱碘酶活性.结果显示,TBBPA能使红鲫血清TT4和TT3水平降低,rT3水平、肝脏脱碘酶ID2和ID3活性升高.PBP对红鲫甲状腺系统的干扰要小于TBBPA,它对红鲫血清TT3水平和ID2活性几乎没有影响.上述结果表明,TBBPA和PBP能够干扰红鲫体内甲状腺激素的稳态,加速甲状腺激素在肝脏内的转化和代谢;这可能是通过抑制甲状腺激素与运载蛋白的结合来实现的.To test the thyroid disrupting effects of brominated flame ratardants in vivo, goldfish （Carassius auratus） were exposed to 0.5 mg· L ^-1 tetrabromobisphenol-A （TBBPA） and 0. 1 mg· L^-1 pentabromophcnol （PBP） singly for 28 d. Plasma thyroid hormone levels and liver deiodinase activities were detected using microparticle enzyme immunoassay and radioimmunoassay. The results showed that TBBPA could decrease plasma TT4 and TT3 levels, and increase plasma rT3 level and liver IDs activities. The disturbance of PBP was less than that of TBBPA. No effects were observed on the plasma TT3 level or liver ID2 activity in groups exposed to PBP. It was deduced that TBBPA and PBP could interfere with the homeostasis of fish thyroid hormones, which might arise from interference with the binding of thyroid hormones to transthyretin.

关 键 词：四溴双酚-A(TBBPA) 五溴酚(PBP) 甲状腺激素(THs) 脱碘酶(IDs) 红鲫 

分 类 号：X171.5[环境科学与工程—环境科学]