茜素红褪色法测定血红蛋白  

Fading Spectrophotometic Method for the Determination of Hemoglobin with Alizarin Red

作　　者：陈亚红[1] 卜彦林[2] 田丰收[1] 

机构地区：[1]周口师范学院化学系,河南周口466000 [2]河南省水利水电学校,河南周口466001

出　　处：《信阳师范学院学报（自然科学版）》2008年第3期436-438,共3页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：中国博士后科学基金资助项目(20070410876);河南省教育厅自然科学基金资助项目(2008A150029)

摘　　要：在碱性介质中,H2O2产生的羟自由基可以氧化茜素红使之褪色,而过氧化物模拟酶——血红蛋白能够加速茜素红的褪色程度.基于血红蛋白对H2O2氧化茜素红体系的催化作用,建立了一种方便、快捷、灵敏的测定血红蛋白的催化动力学新方法.探讨了测定血红蛋白的最佳条件和干扰情况.该方法测定的线性范围为1.5×10-9～5.0×10-8mol/L,方法的检出限为6.0×10-10mol/L,表观摩尔吸光系数为3.4×106L.mol-1.cm-1.该方法已成功地应用于动物血液中血红蛋白含量的测定,结果满意.A simple spectrometric method was developed for the determination of hemoglobin based on it＇s catalytic activity on oxidation of alizarin red in presence of hydrogen peroxide, The optimum conditions and effects of foreign ions were investigated. The results indicated that hemoglobin concentration demonstrated good linear correlation in the range of 1.5 ×10^-9～5, 0 ×10^-8 moL/L, and the apparent molar absorptivity ε532 is 3.4 ×10^6 L · mol^-1·cm^-1 with a detection limit of 6.0 ×10^-10mol/L. This method has been applied to the determination of hemoglobin concentration in blood samples with satisfactory results.

关 键 词：血红蛋白 茜素红 过氧化氢 分光光度法 

分 类 号：O657.32[理学—分析化学]