生物信息学技术分析并克隆超级稻生长素结合蛋白cDNA  

Molecular Analysis and Cloning of Rice Auxin-binding Proteinl cDNA by Bioinformatics Strategy

作　　者：程鹏[1] 蒋泓[2] 欧阳琳[3] 王若仲[1] 肖浪涛[1] 

机构地区：[1]湖南农业大学湖南省植物激素与生长发育重点实验室,湖南长沙410128 [2]佛山科学技术学院,广东佛山528000 [3]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《农业现代化研究》2008年第4期506-509,共4页Research of Agricultural Modernization

基　　金：国家自然科学基金<植物激素;植酸与杂交水稻籽粒灌浆的关系及调控的研究>(编号:30600049)

摘　　要：为进一步研究超级稻的生长素结合蛋白,采用生物信息学方法获得其全长cDNA序列,并进行分析;对全长序列进行了全长验证。从数据库搜索获得了一条水稻cDNA序列,含一个推测的206个氨基酸的开放阅读框,与其它植物的ABP1cDNA序列和ABP1蛋白质序列具有明显的同源性,并且其推测编码的蛋白质的各种性质,类似于其它植物的ABP1。结果显示,该序列为水稻的ABP1cDNA。以该序列为指导,利用RT-PCR扩增超级稻ABP1cDNA,并克隆测序,其序列与电子搜索结果一致。In order to study super rice auxin-binding protein 1, its full-length cDNA sequence was obtained and analyzed by bioinforrnatics and laboratory bench-work. In the results, a rice cDNA sequence was got from nucleotide sequence database, the putative open reading frame of the sequence encodes a presumptive protein containing 206 amino acid residues. Blast against the current GenBank DNA and protein sequence database reveal significant homology with the ABP 1 of other plants, and the functions of presumptive protein resemble these of the ABP 1 of other plants. These results suggest the sequence is rice ABP1cDNA. Based the sequence, ABP1cDNA of super rice was amplified with RT-PCR and cloned for sequencing, the result of sequencing accord with electronic seaching outcome.

关 键 词：生物信息学 超级稻 生长素结合蛋白 克隆 

分 类 号：Q785[生物学—分子生物学]