转豇豆胰蛋白酶抑制剂基因抗虫甘蓝植株的获得  

AN INSECT-RESISTANT TRANSGENIC CABBAGE PLANT WITH COWPEA TRYPSIN INHIBITOR (CpTI) GENE

作　　者：方宏筠[1] 李大力 王关林[1] 李英慧[1] 朱祯[2] 李向辉[2] 

机构地区：[1]辽宁师范大学生物工程研究所,大连116029 [2]中国科学院遗传研究所,北京100101

出　　处：《Acta Botanica Sinica》1997年第10期940-945,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：辽宁省科委重点资助项目

摘　　要：利用根癌农杆菌(Agrobacterium tumefaciens)介导,将豇豆胰蛋白酶抑制剂(CpTI)基因导入甘蓝(Brassica oleracea var.capitata L.)栽培品种“京丰”和“迎春”,并分别获得了转基因再生植株。对NPTⅡ的ELISA检测表明,标记基因NPTⅡ已在再生植株中得到有效表达。对转化植株进行Southern blot分子杂交实验进一步证明CpTI基因已整合到甘蓝的基因组。实验室内的叶片离体饲虫及盆栽饲虫试验表明,转基因甘蓝对菜青虫具有一定抗性。实验中还观察到,采用愈伤组织分化途径可以获得较高的转化频率,但嵌合体较多;而直接分芽的途径转化率低,但嵌合体较少。Cowpea trypsin inhibitor (CpTⅠ) gene was transformed into Brassica oleracea var. capi-tata variety 'Yingchun' and 'Jingfeng' mediated by the Agrobacterium tumefaciens LBA4404 (pRCL27). Transgenic plants were obtained from transformed calli or explants. It was shown from the ELISA assay that NPT Ⅱ gene was expressed in the transgenic cabbage celk. The integration of the CpTⅠ gene into cabbage genome DNA was confirmed by Southern blotting. Insect-tolerance of the transgenic plants to Pieris rapae L. was observed by bioassays on the transgenic plants in the laboratory.

关 键 词：豇豆 胰蛋白酶 抑制剂基因 转基因植株 抗虫 

分 类 号：S643.403.2[农业科学—蔬菜学] S188[农业科学—园艺学]