大鼠侧脑室及鞘内注射孤啡肽对痛反应及电针镇痛的影响  

EFFECT 0F INTRATHECAL OR INTRACEREBROVENTRICULAR ADMINISTRITION OF 0FQ 0N PAIN THRESH0LD AND ACPUNCTURE ANALGESIA IN RATS

作　　者：张秀琳[1] 朱崇斌[1] 许绍芬[1] 曹小定[1] 吴根诚[1] 李默漪[2] 崔大敷 戚正武[2] 

机构地区：[1]上海医科大学医学神经生物学国家重点实验室 [2]中国科学院上海生物化学研究所,上海200031

出　　处：《生理学报》1997年第5期575-580,共6页Acta Physiologica Sinica

基　　金：国家自然科学基金!39670901;上海市科委基金!95×D14004;上海市科技启明星计划资助

摘　　要：孤啡肽（orphaninFQ，OFQ）是最近发现的一种门肽，为阿片受体家族中一个新成员—“孤儿受体”（orphareceptor）的内源性配基。OFQ在痛觉调制中的作用与内阿片肽明显不同。本实验在大鼠电刺激甩尾测痛模型上，观察了侧脑室（ICV）及鞘内注射（IT）OFQ对伤害性电刺激引起的痛反应及电针镇痛的影响。结果发现，大鼠ICV及IT0．1μg（0.055nmol）OFQ对痛反应无影响，而1.0μg（0.55nmol）的OFQ可明显降低痛阈；但两种剂量的0FQ阴无论是脑室还是鞘内给药，均可对抗电针镇痛；采用反义核苷酸技术阻断OFQ受体合成，大鼠痛阈升高，此时再给予OFQ，则电针镇痛效应无明显改变。表明OFQ对基础痛阈的影响与剂量有关：小剂量无影响，大剂量使痛阈降低。但无论对基础痛阈有无影响，OFQ均对抗电针镇痛。Orphanin FQ (OFQ ) is a newly discovered 17-amino-acid peptide capable of inducing hyperalgesia.In the present study, the effects of OFQ on basal pain threshold and acupuncture anlgesia (AA) in rats were observed using the tail-flick tesl. It was found that intrathecal (IT) or intracerebroventricular (ICV ) administrition of 0. 1 μg OFQ had no effect on basal pain threshold of rats, while 1 μg OFQ could lower the threshold. However, OFQ at both the doses (0. 1 or 1 .0 μg) administered by either IT or ICV injection could antagonize AA with that occuring in the brain being more prominent then in the spinal cord. When the rats were repeatedly treated with antisense oligonucleotide to block synthesis of OFQ receptor, pain threshold increased significantly.At such instance, when the OFQ was combined with acupuncture, the effect of AA showed no obvious change. The above results show that the OFQ at small dose has no effect on pain threshold but can lower it at larger d0se; while in both cases OFQ can antagonize AA.

关 键 词：孤啡肽 痛觉调制 针刺镇痛 

分 类 号：R971[医药卫生—药品] R246.2[医药卫生—药学]