硝基左旋精氨酸对睡眠抑制作用的机制研究  

A STUDY OF THE MECHANISM OF L-NNA ON SLEEP INHIBITI0N

作　　者：章茜[1] 王书春[1] 乔鹏[1] 胡群圆 陈力超[1] 王雨若[1] 

机构地区：[1]河南医科大学生理教研究室,郑州450052 [2]河南职工医学院组织胚胎教研室,郑州450003

出　　处：《生理学报》1997年第5期585-588,共4页Acta Physiologica Sinica

摘　　要：本文观察了硝基左旋精氨酸（L-NNA，50mg／kg，ip）和L-精氨酸（L-arg，110mg/kg，ip）对慢性植入电极的大鼠睡眠-觉醒周期的影响及中缝核5-羟色胺（5-HT）神经元免疫阳性反应的变化。结果表明：L-NNA显著抑制慢波睡眠和快眼动睡眠，使平均动脉压（MAP）升高。L-arg则使MAP显著降低，对睡眠无明显影响。预先给予L-arg可逆转L-NNA的效应。腹腔给予L-NNA后2h，中缝背核（DR）和中央上核（MnR）5-HT神经元免疫阳性细胞明显增多，与对照比较，P＜0.01，此效应可被预先给予L-arg所减弱。提示L-arg：NO通路参与睡-醒周期节律的调节。中缝核头部5-HT神经元免疫阳性反应增强可能与L-NNA抑眠作用有关。In the present study, the sleep-waking cycle and the immunoreactivity of 5-HTcontainingneurons in rats receiving intraperitoneal injections of N-Nitro-L-arginine (L-NNA, 50 mg/kg) and L-arginine (L-arg, 110 mg/kg) were observed respectively.The results showed that injection of L-NNA suppressed both slow wave sleep (SWS) and rapid eye movement sleep (REMS) and increased mean arterial pressure (MAP). L-arg markedly attenuated MAP, whereas SWS and REMS were unchanged. The effects of L-NNA on sleep and MAP were reversed by preinjection of L-arg. In comparison with control group, the positive cells in dorsal raphe nucleus (DR) and raphe magUs nucleus (MnR ) increased 2 h a.fer injection of L-NNA. The effect was attenuated by preadministration of L-arg. All these results suggest that L-arg: NO pathway is involved in theregUlation of sleep-waking cycle and the increment of the positive immunoreactivity of the 5-HT-c0ntaining neurons in the DR may be related to the sleep-suppressive effects induced by L-NNA.

关 键 词：睡眠 硝基左旋精氨酸 生理学 

分 类 号：R338.63[医药卫生—人体生理学]