猪生长激素基因表达质粒的构建及其转基因动物研究  

The Construction of Porcine Growth Hormone Gene Expressive

作　　者：陈清轩[1] 曹颉[1] 宋德秀 陈永福[2] 魏庆信[3] 

机构地区：[1]中国科学院发育生物学研究所 [2]北京农业大学生物学院 [3]湖北省农科院畜牧兽医研究所

出　　处：《生物化学杂志》1997年第5期540-545,共6页

基　　金：国家"863"计划

摘　　要：将猪生长激素基因（ＰＧＨ）克隆到质粒ｐＵＣ１９上，经酶切分析，确定其酶切图谱．把ＰＧＨ转录起始位点以前的序列切掉，换上羊ＭＴ－１ａ基因的启动子，构建成可以调控的表达载体ｐＳＭＴＰＧＨ，用于转基因动物的研究．采用微注射法将线状ｐＳＭＴＰＧＨ导入猪、鼠和金鱼的受精卵中，得到了相应的转基因动物．对这些动物鉴定分析表明，外源基因整合率因动物不同而异，但该基因在这３种动物中的整合率均在９％以上，其生长速度都高于对照组．The expressive plasmid pSMTPGH was constructed with porcine growth hormone gene (PGH) and sheep metallothionein promoter (SMT).The linear fusion gene constructs were microinjected into zygots of mice,pigs and gold fishes separately.Three different transgenic animals were produced,in which the foreign gene constructs were intergrated at the rate of over 9 0%.The average growth rate of the transgenic animals are higher than control animals (normal animals).These results demonstrate that the production of stable lines of transgenic farm aninals with enhanced growth performances is feasible.

关 键 词：猪 生长激素基因 表达载体 转基因动物 质粒 

分 类 号：S828.2[农业科学—畜牧学] S188[农业科学—畜牧兽医]