藻胆蛋白复合物的合成及其分子内能量传递  

The Synthesis of Phycobiliprotein Conjugates and Their Intramolecular Energy Transfer Phenomena

作　　者：赵继全[1] 赵井泉[1] 杨紫萱[1] 张建平[1] 蒋丽金[1] 

机构地区：[1]中国科学院感光化学研究所,北京100101

出　　处：《生物化学与生物物理进展》1997年第5期435-440,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助! (396 70 0 6 5 )

摘　　要：通过偶联剂 3 ( 2 吡啶联巯基 )丙酸N 羟基琥珀亚胺酯 (SPDP)及改变配料比 ,合成了两个R 藻红蛋白 (R PE)与C 藻蓝蛋白 (C PC)的复合物A和B .利用吸收光谱确定了分子内R PE与C PC的摩尔比为 6∶1和 2∶1 .通过荧光光谱 ,观察到能量传递现象 ,并计算出能量传递效率为 63%和88% .证明分子内能量传递效率很高 .二硫苏糖醇 (DTT)还原连接R PE与C PC的二硫桥键后 ,能量传递被阻断 .这一现象进一步证明复合物中存在分子内能量传递 .Two conjugates of R-phycoerythrin (R-PE) and C-phycocyanin (C-PC) were synthesized through a coupling agent, N- succinmidyl 3-(2-pyridyldithio)-propionate and the alternation of the ratio of R-PE to C-PC. Their ratio of R-PE to C-PC were determined by absorption spectra. One is 6∶1, the other is 2∶1. The phenomena of intramolecular energy transfer were characterized. The calculated results showed that the efficiency of energy transfer from R-PE to C-PC was 63% and 88% respectively. The efficiency of energy transfer is better. When the disulfide bridge between R-PE and C-PC was reduced by DTT, the energy transfer was stopped. The phenomena further verified the intramolecular energy transfer from R-PE to C-PC.

关 键 词：藻胆蛋白 蛋白复合物 合成 能量传递 

分 类 号：Q949.203[生物学—植物学]