水-有机溶剂两相体系中甲基单胞菌Z201催化丙烯环氧化的初步研究  

Preliminary Study on the Epoxidation of Propene by Methylomonas Z201 Cells in Water organic Solvent Two phase System

作　　者：夏仕文[1] 尉迟力[1] 李树本[1] 

机构地区：[1]中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室

出　　处：《生物工程学报》1997年第4期446-449,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金

摘　　要：水有机溶剂两相体系中甲基单胞菌Ｚ２０１催化丙烯环氧化的初步研究夏仕文尉迟力李树本（中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室兰州７３００００）Ｌａｎｎｅ于１９８７年提出了生物催化剂工程（Ｂｉｏｃａｔａｌｙｓｔｅｎｇｉｎｅｒｉ...In water organic solvent two phase systems, the activity retention of free, absorbed and entrapped Methylomonas Z201 cells was depended on the polarity of solvents. Correlation of activity retention solvent hydrophobicity (Log P ) revealed that the activity retention was high in solvents with Log P >4. In water hexadecane(10%, v/v ) two phase system, lower volumetric phase ratio of hexadecane in reaction medium and higher stirring rate were favorable for the increase of reaction rate of propene. Although propene oxide produced could be partly extracted into hexadecane phase, the limited extraction capacity of hexadecane was not enough to decrease the concentration of propene oxide in aqueous phase to the inhibition concentration, the operational stability of Methylomonas Z201 cells could not be enhanced.

关 键 词：生物工程 水-有机溶剂 甲基单胞菌 丙烯环氧化 

分 类 号：Q81[生物学—生物工程]