高效液相色谱法测定乳病消片中丹参酮ⅡA含量  

Determination of tanshionone ⅡA in Rubingxiao tablets by HPLC

作　　者：李红卫[1] 荆晓红[1] 张晓琴[2] 

机构地区：[1]兰州大学第二医院药剂科,甘肃兰州730030 [2]兰州大学药学院,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2008年第2期47-49,共3页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的建立对乳病消片中丹参酮ⅡA的含量测定方法。方法色谱柱:Zorbax SB-C_(18)(4.6 mm×15 cm);流动相:V(甲醇):V(水)=81:19;流速:1.0 mL/min;检测波长:270 nm;柱温:30℃。结果丹参酮ⅡA在1.96～47.04μg/mL范围内线性关系良好,回收率试验结果满意,重复性好。结论本法简便、准确,可以用于制剂质量控制。Objective To establish an HPLC method for determination of tanshionone ⅡA in Rubingxiao tablets. Methods The column was Zorbax SB-C18（4.6 mm×15 cm）, the mobile phase consisted of V（methanol） ： V（water）=81 ： 19 at the flow rate of 1 mL/min, the wavelength was set at 270 nm and the column temperature was 30℃. Results In this condition there was a good linear relationship over the concentration range of 1.96-47.04 μg/mL for tanshionone ⅡA. Conclusion This method is simple and accurate, it could be applied to the determination of the tablets.

关 键 词：乳病消片 高效液相色谱法 丹参酮ⅡA 含量测定 

分 类 号：R927.2[医药卫生—药学]