双(α-呋喃甲酸)氧钒对胰岛素抵抗3T3-L1脂肪细胞糖摄取的影响  

Effects of bis (α-furancarboxylato) oxovanadium (IV) on the glucose uptake in normal and insulin-resistant 3T3-L1 adipocytes

作　　者：左益檠[1] 牛艳芬[1] 林华[1] 王芳[1] 刘伟平[2] 李玲[1] 

机构地区：[1]昆明医学院云南省天然药物药理重点实验室,云南昆明650031 [2]昆明贵金属研究所,云南昆明650221

出　　处：《中国药理学通报》2008年第7期902-905,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30260118);云南省应用基础研究计划重点资助项目(No2006C009Z)

摘　　要：目的探讨新型的有机羧酸氧钒配合物双（α-呋哺甲酸）氧钒（BFOV）对正常及胰岛素抵抗的3T3-L1脂肪细胞糖摄取的影响。方法采用地塞米松诱导313-L1脂肪细胞建立胰岛素抵抗的细胞模型，研究双（d-呋喃甲酸）氧钒对正常及胰岛素抵抗313-L1脂肪细胞葡萄糖消耗的影响。结果双（d-呋喃甲酸）氧钒（2．5μmol·L^-1 - 40μmol·L^-1）对正常的313-L1脂肪细胞仅有增加葡萄糖消耗量的趋势，与空白对照组比较，差异无显著性；但能明显增加地塞米松诱导的胰岛素抵抗313-L1脂肪细胞的葡萄糖消耗量，改善模型细胞的胰岛素抵抗状态。结论双（d-呋喃甲酸）氧钒能促进胰岛素抵抗脂肪细胞的葡萄糖摄取，改善胰岛素抵抗状态。Aim To investigate the effects of bis [（α-furancarboxylato） oxovanadium （IV） , BFOV] on the glucose uptake in normal and insulin-resistant 3T3-L1 adipocytes. Method Fully differentiated 3T3-L1 adipocytes were treated with dexamethasone to induce insulin resistance. Effects of bis （α-furancarboxylato） oxovanadium （IV） on the glucose uptake in normal and insuhn-resistant adipocytes were estimated by the chan-ges of glucose consumption in the medium measured by the glucose oxidase method. Results BFOV （2.5-40 ）μmol·L^-1） produced the tendency of increasing the glucose consumption in normal cells, compared with untreated control cells （P〉0.05 ）. However, BFOV markedly enhanced the glucose consumption in insulinresistant 3T3-L1 adipocytes, resulting in the improvement of insulin resistance induced by dexamethasone. Conclusion BFOV increased the glucose uptake in insulin-resistant adipocytes, suggesting the improvement of insulin resistance.

关 键 词：双(Α-呋喃甲酸)氧钒 313-L1脂肪细胞 地塞米松 胰岛素抵抗 葡萄糖消耗 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R329.24[医药卫生—基础医学]